Effect of low-level laser therapy on abdominal adipocytes before lipoplasty procedures

Spencer A. Brown, Rod J. Rohrich, Jeffrey Kenkel, V. Leroy Young, John Hoopman, Maria Coimbra

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Low-level laser therapy is a new subspecialty for the medical application of lasers that provides therapeutic rather than surgical outcomes for many medical indications. Recently, low-level laser therapy was reported to "liquefy" or release stored fat in adipocytes by the opening of specialized yet not identified cell membrane-associated pores after a brief treatment. Currently, low-level laser therapy is a U.S. Food and Drug Administration-approved technology for improving pain alleviation. To explore these data further, a series of in vitro studies on human preadipocytes and institutional animal care and use committee-approved protocols in a porcine Yucatan model and an institutional review board-approved clinical study were performed. Using a 635-nm low-level laser of 1.0 J/cm2 supplied to the authors by the vendor, these studies were designed to determine whether alteration in adipocyte structure or function was modulated after low-level laser therapy. Cultured human preadipocytes after 60 minutes of laser therapy did not change appearance compared with nonirradiated control cells. In the porcine model, low-level laser therapy (30 minutes) was compared with traditional lipoplasty (suction-assisted lipoplasty) and ultrasound-assisted lipoplasty. From histologic and scanning electron microscopic evaluations of the lipoaspirates, no differences were observed between low-level laser therapy-derived and suction-assisted lipoplasty-derived specimens. Using exposure times of 0, 15, 30, and 60 minutes in the presence or absence of superwet wetting solution and in the absence of lipoplasty, total energy values of 0.9 mW were delivered to tissue samples at three increasing depths from each experimental site. No histologic tissue changes or specifically in adipocyte structure were observed at any depth with the longest low-level laser therapy (60 minutes with superwet fluid). Three subjects under-going large-volume lipoplasty were exposed to superwet wetting fluid infiltration 14 minutes before and 12 minutes after, according to vendor instructions. Tissue samples from infiltrated areas were collected before suction-assisted lipoplasty and lipoaspirates from suction-assisted lipoplasty. No consistent observations of adipocyte disruptions were observed in the histologic or scanning electron microscopy photographs. These data do not support the belief that low-level laser therapy treatment before lipoplasty procedures disrupts tissue adipocyte structure.

Original languageEnglish (US)
Pages (from-to)1796-1804
Number of pages9
JournalPlastic and Reconstructive Surgery
Volume113
Issue number6
DOIs
StatePublished - May 2004

Fingerprint

Lipectomy
Adipocytes
Suction
Lasers
Swine
Animal Care Committees
Low-Level Light Therapy
Research Ethics Committees
Laser Therapy
United States Food and Drug Administration
Electron Scanning Microscopy
Therapeutics
Fats
Cell Membrane
Electrons
Technology
Pain

ASJC Scopus subject areas

  • Surgery

Cite this

Effect of low-level laser therapy on abdominal adipocytes before lipoplasty procedures. / Brown, Spencer A.; Rohrich, Rod J.; Kenkel, Jeffrey; Young, V. Leroy; Hoopman, John; Coimbra, Maria.

In: Plastic and Reconstructive Surgery, Vol. 113, No. 6, 05.2004, p. 1796-1804.

Research output: Contribution to journalArticle

Brown, Spencer A. ; Rohrich, Rod J. ; Kenkel, Jeffrey ; Young, V. Leroy ; Hoopman, John ; Coimbra, Maria. / Effect of low-level laser therapy on abdominal adipocytes before lipoplasty procedures. In: Plastic and Reconstructive Surgery. 2004 ; Vol. 113, No. 6. pp. 1796-1804.
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