TY - JOUR
T1 - Effect of luminal angiotensin II on rabbit proximal convoluted tubule bicarbonate absorption
AU - Baum, Michel
AU - Quigley, Raymond
AU - Quan, Albert
PY - 1997/10
Y1 - 1997/10
N2 - The present in vitro microperfusion study examined the effect of luminal angiotensin II on proximal convoluted tubule (PCT) volume absorption and bicarbonate transport. Neither 10-11 M, 10-10 M, nor 2 x 10-8 M luminal angiotensin II significantly affected PCT transport. When tubules were first perfused with enalaprilat to inhibit endogenous angiotensin II production, addition of 10-10 M luminal angiotensin II increased volume absorption (0.72 ± 0.08 vs. 0.86 ± 0.07 nl · mm-1 · min-1, P < 0.01) and bicarbonate transport (52.3 ± 3.7 vs. 67.9 ± 4.2 pmol · mm-1 · min-1, P < 0.01). Addition of 10-6 M losartan, an AT1 inhibitor, to the luminal perfusate inhibited volume absorption (0.95 ± 0.14 vs. 0.72 ± 0.11 nl · mm-1 · min-1, P < 0.05) and bicarbonate transport (65.0 ± 7.3 vs. 54.7 ± 9.2 pmol · mm-1 · min-1, P < 0.05). Addition of 10-4 M luminal PD-123319, an AT2 inhibitor, was without effect. In tubules perfused with 10-4 M luminal enalaprilat and 10-4 M luminal PD-123319, addition of 10-10 M luminal angiotensin II in the experimental period resulted in a stimulation in volume absorption (0.61 ± 0.08 vs. 0.81 ± 0.10 nl · mm-1 · min-1, P < 0.01) and bicarbonate transport (49.9 ± 6.3 vs. 77.4 ± 14.3 pmol · mm-1 · min-1, P < 0.01). In tubules perfused with 10-6 M losartan and 10-4 M enalaprilat, addition of luminal 10-10 M angiotensin II resulted in no change in transport. These data are consistent with endogenous angiotensin II affecting PCT bicarbonate transport in vitro via luminal AT1 receptors.
AB - The present in vitro microperfusion study examined the effect of luminal angiotensin II on proximal convoluted tubule (PCT) volume absorption and bicarbonate transport. Neither 10-11 M, 10-10 M, nor 2 x 10-8 M luminal angiotensin II significantly affected PCT transport. When tubules were first perfused with enalaprilat to inhibit endogenous angiotensin II production, addition of 10-10 M luminal angiotensin II increased volume absorption (0.72 ± 0.08 vs. 0.86 ± 0.07 nl · mm-1 · min-1, P < 0.01) and bicarbonate transport (52.3 ± 3.7 vs. 67.9 ± 4.2 pmol · mm-1 · min-1, P < 0.01). Addition of 10-6 M losartan, an AT1 inhibitor, to the luminal perfusate inhibited volume absorption (0.95 ± 0.14 vs. 0.72 ± 0.11 nl · mm-1 · min-1, P < 0.05) and bicarbonate transport (65.0 ± 7.3 vs. 54.7 ± 9.2 pmol · mm-1 · min-1, P < 0.05). Addition of 10-4 M luminal PD-123319, an AT2 inhibitor, was without effect. In tubules perfused with 10-4 M luminal enalaprilat and 10-4 M luminal PD-123319, addition of 10-10 M luminal angiotensin II in the experimental period resulted in a stimulation in volume absorption (0.61 ± 0.08 vs. 0.81 ± 0.10 nl · mm-1 · min-1, P < 0.01) and bicarbonate transport (49.9 ± 6.3 vs. 77.4 ± 14.3 pmol · mm-1 · min-1, P < 0.01). In tubules perfused with 10-6 M losartan and 10-4 M enalaprilat, addition of luminal 10-10 M angiotensin II resulted in no change in transport. These data are consistent with endogenous angiotensin II affecting PCT bicarbonate transport in vitro via luminal AT1 receptors.
KW - Angiotensin receptor
KW - DuP- 753
KW - In vitro microperfusion
KW - Losartan
KW - PD-123319
KW - Volume absorption
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U2 - 10.1152/ajprenal.1997.273.4.f595
DO - 10.1152/ajprenal.1997.273.4.f595
M3 - Article
C2 - 9362337
AN - SCOPUS:0030703598
SN - 1931-857X
VL - 273
SP - F595-F600
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 4 42-4
ER -