Effect of metal ions on the binding of gentamicin to the peptidoglycan of Micromonospora echinospora

Ju Chu, Wenze Niu, Siliang Zhang, Yingping Zhuang, Hui Hu, Yourong Li

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Peptidoglycan (PG), the major part of the cell wall (CW) of Micromonospora echinospora var 49-925, could bind about 90% of the total gentamicin (GM) absorbed on the CW and when the concentrations of PG and GM were 6 mg/ml and 500 U/ml, respectively, the amount of bound GM was approximately 88 000 U/g of PG (dry weight) and no free GM was detected in the solution. Metal ions were used to release GM from PG. Mg2+ and Na+ restrained intensively the binding of GM at GM concentration less than 1000 U/ml. Amine could compete with GM for the binding sites as well. About 50% increase of GM titre was achieved when Mg2+ or Na+ was supplemented to the fermentation broth.

Original languageEnglish (US)
Pages (from-to)1145-1150
Number of pages6
JournalProcess Biochemistry
Volume39
Issue number9
DOIs
StatePublished - May 31 2004

Keywords

  • Gentamicin
  • Metal ions
  • Micromonospora echinospora
  • Peptidoglycan

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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