Abstract
Peptidoglycan (PG), the major part of the cell wall (CW) of Micromonospora echinospora var 49-925, could bind about 90% of the total gentamicin (GM) absorbed on the CW and when the concentrations of PG and GM were 6 mg/ml and 500 U/ml, respectively, the amount of bound GM was approximately 88 000 U/g of PG (dry weight) and no free GM was detected in the solution. Metal ions were used to release GM from PG. Mg2+ and Na+ restrained intensively the binding of GM at GM concentration less than 1000 U/ml. Amine could compete with GM for the binding sites as well. About 50% increase of GM titre was achieved when Mg2+ or Na+ was supplemented to the fermentation broth.
Original language | English (US) |
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Pages (from-to) | 1145-1150 |
Number of pages | 6 |
Journal | Process Biochemistry |
Volume | 39 |
Issue number | 9 |
DOIs | |
State | Published - May 31 2004 |
Keywords
- Gentamicin
- Metal ions
- Micromonospora echinospora
- Peptidoglycan
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology