Effect of Naftifine on Neutrophil Adhesion

Tak Shun Choi, Barry Solomon A Maja Nowakowski, Wei Li Lee, Susan Geen, Katnala Suntharalingam, Senih Fikrig, Alan R. Shalita

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Effective methods of fungal treatment involve reduction in fungal infections and host inflammatory responses. Naftifine (NF), a topical antifungal agent, is highly active in vitro and in vivo against a wide range of pathogenic fungi. Additionally NF has been shown to inhibit polymorphonuclear leukocyte (PMN) chemotaxis and respiratory burst activity in an irreversible dose-dependent and time-dependent manner. Since leukocyte adherence to endothelia is believed to be one of the initial crucial events in the recruitment of circulating leukocytes to the site of inflammation, we have investigated the in vitro effect of NF on PMN adherence to nylon fiber, BSA-coated glass chamber or polystyrene, and endothelial mono-layers via three adherence assays. All three assays demonstrated a statistically significant reduction (p < 0.01-0.001) in PMN adherence to the respective media. In particular, NF (at 30-60 μg/ml) significantly inhibited PMN adherence to endothelial monolayers (p < 0.01) as measured spectrophotometrically by the uptake of rose bengal stain. Therefore, NF inhibits PMN adherence to endothelia in our in vitro model system. This inhibition may constitute part of the anti-inflammatory effect of NF.

Original languageEnglish (US)
Pages (from-to)190-196
Number of pages7
JournalSkin Pharmacology and Physiology
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Naftifine
  • Neutrophil adhesion

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Dermatology

Fingerprint Dive into the research topics of 'Effect of Naftifine on Neutrophil Adhesion'. Together they form a unique fingerprint.

  • Cite this

    Choi, T. S., Nowakowski, B. S. A. M., Lee, W. L., Geen, S., Suntharalingam, K., Fikrig, S., & Shalita, A. R. (1996). Effect of Naftifine on Neutrophil Adhesion. Skin Pharmacology and Physiology, 9(3), 190-196. https://doi.org/10.1159/000211415