Effect of nitric oxide on release of glutamate in the subretrofacial nucleus (SRF) during the exercise pressor reflex in cats

Jianhua Li, Jere H. Mitchell

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The subretrofacial nucleus (SRF) has been known to play a crucial role in the expression of the exercise pressor reflex. Previously, we have reported that the release of glutamate (Glu) in the SRF was increased during muscle contraction in anesthetized cats. In this study, static muscle contraction of the triceps surae for 4 min was induced by electrical stimulation of L7 and S1 ventral roots. Endogenous release of Glu and citrulline (Cit) from the SRF was recovered by microdialysis and measured by HPLC. The microdialysis probes were also used to deliver L-arginine and L-NAME to test the effect of nitric oxide (NO) on release of Glu in the SRF and on the cardiovascular responses during muscle contraction. During control, muscle contraction significantly increased mean arterial pressure (MAP) from 98±8 to 151±9 mmHg, and the extracellular concentration of Glu from 610±120 to 1280±290 nM. Dialyzing 2 mM L-arginine into the SRF increased basal Cit concentration from 260±50 to 760±210 nM (P<0.05). During contraction after L-arginine, the increases in MAP and Glu concentration were significantly attenuated (86±3-124±6 mmHg and 300±60-460±100 nM, respectively). Dialysis of 0.5 mM L-NAME into the SRF decreased Cit concentration from 340±40 to 180±20 nM (P<0.05). During contraction after dialyzing L-NAME, the increases in MAP and Glu concentration were significantly potentiated (93±6-154±9 mmHg and 520±80-1290±380 nM, respectively). These results suggest that endogenous NO modulates the cardiovascular responses to static muscle contraction by affecting the release of Glu in the SRF.

Original languageEnglish (US)
Pages (from-to)195-202
Number of pages8
JournalBrain Research
Volume950
Issue number1-2
DOIs
StatePublished - Sep 20 2002

Fingerprint

Reflex
Glutamic Acid
Nitric Oxide
Cats
Muscle Contraction
Citrulline
NG-Nitroarginine Methyl Ester
Arginine
Arterial Pressure
Microdialysis
Spinal Nerve Roots
Basal Ganglia
Electric Stimulation
Dialysis
High Pressure Liquid Chromatography

Keywords

  • Blood pressure
  • Excitatory amino acid
  • Glutamate
  • Microdialysis
  • Muscle contraction
  • Nitric oxide
  • Subretrofacial nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of nitric oxide on release of glutamate in the subretrofacial nucleus (SRF) during the exercise pressor reflex in cats. / Li, Jianhua; Mitchell, Jere H.

In: Brain Research, Vol. 950, No. 1-2, 20.09.2002, p. 195-202.

Research output: Contribution to journalArticle

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abstract = "The subretrofacial nucleus (SRF) has been known to play a crucial role in the expression of the exercise pressor reflex. Previously, we have reported that the release of glutamate (Glu) in the SRF was increased during muscle contraction in anesthetized cats. In this study, static muscle contraction of the triceps surae for 4 min was induced by electrical stimulation of L7 and S1 ventral roots. Endogenous release of Glu and citrulline (Cit) from the SRF was recovered by microdialysis and measured by HPLC. The microdialysis probes were also used to deliver L-arginine and L-NAME to test the effect of nitric oxide (NO) on release of Glu in the SRF and on the cardiovascular responses during muscle contraction. During control, muscle contraction significantly increased mean arterial pressure (MAP) from 98±8 to 151±9 mmHg, and the extracellular concentration of Glu from 610±120 to 1280±290 nM. Dialyzing 2 mM L-arginine into the SRF increased basal Cit concentration from 260±50 to 760±210 nM (P<0.05). During contraction after L-arginine, the increases in MAP and Glu concentration were significantly attenuated (86±3-124±6 mmHg and 300±60-460±100 nM, respectively). Dialysis of 0.5 mM L-NAME into the SRF decreased Cit concentration from 340±40 to 180±20 nM (P<0.05). During contraction after dialyzing L-NAME, the increases in MAP and Glu concentration were significantly potentiated (93±6-154±9 mmHg and 520±80-1290±380 nM, respectively). These results suggest that endogenous NO modulates the cardiovascular responses to static muscle contraction by affecting the release of Glu in the SRF.",
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