Effect of postnatal maternal protein intake on prenatal programming of hypertension

Khurrum Siddique, German Lozano Guzman, Jyothsna Gattineni, Michel Baum

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study examined whether postnatal maternal dietary protein deprivation during the time of nursing can program hypertension when the offspring are studied as adults. Rats were fed either a 6% or 20% protein diet during the second half of pregnancy and continued on the same diet while rats were nursing their pups. The neonates of all of the rats were cross-fostered to a different mother and studied as adults. Adult rats that had a normal prenatal environment but were reared by mothers fed a low-protein diet until weaning (20%-6%) were hypertensive, had a higher renal Na+-K+-2Cl′ cotransporter (NKCC2) and Na+-Cl′ cotransporter (NCC) protein abundance yet a comparable number of glomeruli, and had higher plasma renin and angiotensin II levels compared to control (20%-20%). Rats whose mothers were fed a 6% protein diet and cross-fostered to a different rat fed a 6% protein diet until weaning (6%-6%) were hypertensive, had elevated plasma renin and angiotensin II levels, and had a reduction in nephron number but had NKCC2 and NCC levels comparable to 20% to 20% offspring. The 6% to 20% had blood pressure and glomerular numbers comparable to 20% to 20% rats. The hypertension resulting from prenatal dietary protein deprivation can be normalized by improving the postnatal environment. Combined prenatal and postnatal maternal dietary protein deprivation and maternal dietary protein deprivation while nursing alone (20%-6%) results in hypertension, but the mechanism for the hypertension in these groups is different.

Original languageEnglish (US)
Pages (from-to)1499-1507
Number of pages9
JournalReproductive Sciences
Volume21
Issue number12
DOIs
StatePublished - Dec 20 2014

Fingerprint

Embryonic and Fetal Development
Mothers
Hypertension
Dietary Proteins
Member 3 Solute Carrier Family 12
Proteins
Diet
Nursing
Weaning
Renin
Angiotensin II
Member 1 Solute Carrier Family 12
Maternal Deprivation
Protein-Restricted Diet
Nephrons
Newborn Infant
Blood Pressure
Kidney
Pregnancy

Keywords

  • barker hypothesis
  • blood pressure
  • dietary protein deprivation
  • glomerular number
  • postnatal programming

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Effect of postnatal maternal protein intake on prenatal programming of hypertension. / Siddique, Khurrum; Guzman, German Lozano; Gattineni, Jyothsna; Baum, Michel.

In: Reproductive Sciences, Vol. 21, No. 12, 20.12.2014, p. 1499-1507.

Research output: Contribution to journalArticle

@article{56429f4179b34600bdbb01945dc8f641,
title = "Effect of postnatal maternal protein intake on prenatal programming of hypertension",
abstract = "This study examined whether postnatal maternal dietary protein deprivation during the time of nursing can program hypertension when the offspring are studied as adults. Rats were fed either a 6{\%} or 20{\%} protein diet during the second half of pregnancy and continued on the same diet while rats were nursing their pups. The neonates of all of the rats were cross-fostered to a different mother and studied as adults. Adult rats that had a normal prenatal environment but were reared by mothers fed a low-protein diet until weaning (20{\%}-6{\%}) were hypertensive, had a higher renal Na+-K+-2Cl′ cotransporter (NKCC2) and Na+-Cl′ cotransporter (NCC) protein abundance yet a comparable number of glomeruli, and had higher plasma renin and angiotensin II levels compared to control (20{\%}-20{\%}). Rats whose mothers were fed a 6{\%} protein diet and cross-fostered to a different rat fed a 6{\%} protein diet until weaning (6{\%}-6{\%}) were hypertensive, had elevated plasma renin and angiotensin II levels, and had a reduction in nephron number but had NKCC2 and NCC levels comparable to 20{\%} to 20{\%} offspring. The 6{\%} to 20{\%} had blood pressure and glomerular numbers comparable to 20{\%} to 20{\%} rats. The hypertension resulting from prenatal dietary protein deprivation can be normalized by improving the postnatal environment. Combined prenatal and postnatal maternal dietary protein deprivation and maternal dietary protein deprivation while nursing alone (20{\%}-6{\%}) results in hypertension, but the mechanism for the hypertension in these groups is different.",
keywords = "barker hypothesis, blood pressure, dietary protein deprivation, glomerular number, postnatal programming",
author = "Khurrum Siddique and Guzman, {German Lozano} and Jyothsna Gattineni and Michel Baum",
year = "2014",
month = "12",
day = "20",
doi = "10.1177/1933719114530186",
language = "English (US)",
volume = "21",
pages = "1499--1507",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "12",

}

TY - JOUR

T1 - Effect of postnatal maternal protein intake on prenatal programming of hypertension

AU - Siddique, Khurrum

AU - Guzman, German Lozano

AU - Gattineni, Jyothsna

AU - Baum, Michel

PY - 2014/12/20

Y1 - 2014/12/20

N2 - This study examined whether postnatal maternal dietary protein deprivation during the time of nursing can program hypertension when the offspring are studied as adults. Rats were fed either a 6% or 20% protein diet during the second half of pregnancy and continued on the same diet while rats were nursing their pups. The neonates of all of the rats were cross-fostered to a different mother and studied as adults. Adult rats that had a normal prenatal environment but were reared by mothers fed a low-protein diet until weaning (20%-6%) were hypertensive, had a higher renal Na+-K+-2Cl′ cotransporter (NKCC2) and Na+-Cl′ cotransporter (NCC) protein abundance yet a comparable number of glomeruli, and had higher plasma renin and angiotensin II levels compared to control (20%-20%). Rats whose mothers were fed a 6% protein diet and cross-fostered to a different rat fed a 6% protein diet until weaning (6%-6%) were hypertensive, had elevated plasma renin and angiotensin II levels, and had a reduction in nephron number but had NKCC2 and NCC levels comparable to 20% to 20% offspring. The 6% to 20% had blood pressure and glomerular numbers comparable to 20% to 20% rats. The hypertension resulting from prenatal dietary protein deprivation can be normalized by improving the postnatal environment. Combined prenatal and postnatal maternal dietary protein deprivation and maternal dietary protein deprivation while nursing alone (20%-6%) results in hypertension, but the mechanism for the hypertension in these groups is different.

AB - This study examined whether postnatal maternal dietary protein deprivation during the time of nursing can program hypertension when the offspring are studied as adults. Rats were fed either a 6% or 20% protein diet during the second half of pregnancy and continued on the same diet while rats were nursing their pups. The neonates of all of the rats were cross-fostered to a different mother and studied as adults. Adult rats that had a normal prenatal environment but were reared by mothers fed a low-protein diet until weaning (20%-6%) were hypertensive, had a higher renal Na+-K+-2Cl′ cotransporter (NKCC2) and Na+-Cl′ cotransporter (NCC) protein abundance yet a comparable number of glomeruli, and had higher plasma renin and angiotensin II levels compared to control (20%-20%). Rats whose mothers were fed a 6% protein diet and cross-fostered to a different rat fed a 6% protein diet until weaning (6%-6%) were hypertensive, had elevated plasma renin and angiotensin II levels, and had a reduction in nephron number but had NKCC2 and NCC levels comparable to 20% to 20% offspring. The 6% to 20% had blood pressure and glomerular numbers comparable to 20% to 20% rats. The hypertension resulting from prenatal dietary protein deprivation can be normalized by improving the postnatal environment. Combined prenatal and postnatal maternal dietary protein deprivation and maternal dietary protein deprivation while nursing alone (20%-6%) results in hypertension, but the mechanism for the hypertension in these groups is different.

KW - barker hypothesis

KW - blood pressure

KW - dietary protein deprivation

KW - glomerular number

KW - postnatal programming

UR - http://www.scopus.com/inward/record.url?scp=84911203551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911203551&partnerID=8YFLogxK

U2 - 10.1177/1933719114530186

DO - 10.1177/1933719114530186

M3 - Article

VL - 21

SP - 1499

EP - 1507

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 12

ER -