Effect of prostaglandins on steroid secretion by human fetal adrenal tissue

In the present investigation we evaluated the effect of prostaglandins on the rate of steroid secretion by human fetal adrenal (HFA) tissue. Prostaglandins F_2α and E₂ (10μg/ml) were added to the culture medium in the presence or absence of ACTH (1 μg/ml). The medium was assayed for content of cortisol (F), dehydroepiandrosterone sulfate (DS) and pregnenolone sulfate (PS) by radioimmunoassay. When HFA tissue fragments were maintained in the absence of ACTH, F secretion was low; PGF but not PGE₂ suppressed F secretion by 60-65%. When ACTH was added to the culture medium, the secretion rate of F increased 15-fold, whereas DS and PS secretion was maintained at or near initial rates of secretion. The addition of PGF_2α to the culture medium containing ACTH resulted in a 80% decrease in F secretion, but PGE₂ only suppressed F secretion by 50%. In contrast, PGE₂ or PGF_2α had little effect on the rate of DS or PS secretion either in the presence or absence of ACTH. In conclusion, prostaglandins appear to inhibit the secretion of F, but not of DS or PS by the HFA.