Cytoplasmic RNA was isolated from cells cultured with LPS and LPS plus a T cell-derived supernatant (SN) (PK 7.1) containing B cell differentiation factors. The steady state levels of isotype-specific mRNA were assessed by Northern blot analysis with γ-specific C(H)3 probes. It was demonstrated that the SN induces an increase in the level of mRNA for γ1 and a concomitant decrease in the levels of mRNA for γ2b and γ3.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Immunology|
|State||Published - 1983|
ASJC Scopus subject areas
- Immunology and Allergy