Effect of the radiolabel mediator tropolone on lymphocyte structure and function

Edward P. Balaban, Theodore R. Simon, Richard G. Sheehan, Eugene P. Frenkel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The in vitro use of the radioisotope indium 111 (111In) was examined as a radiolabel for lymphocytes obtained from both normal individuals and patients with a variety of lymphoid malignancies. Successful cell labeling requires a chelator. The traditional agent, oxine, has proved to be toxic to the lymphoid lineage. Cellular uptake of 111In mediated by the chelator oxine was compared with that of a new chelator, tropolone. Oxine provided better labeling efficiency (48%) than tropolone (35%) for the labeling of normal lymphocytes. By contrast, lymphocytes from patients with chronic lymphocytic leukemia had a nearly twofold greater labeling efficiency when tropolone was substituted for oxine. Further studies demonstrated that tropolone induced functional injury to lymphocytes when mitogenic response to concanavalin A, pokeweed mitogen, and phytohemagglutinin was assessed. Similar toxicity was found when tropolone was compared with oxine. In addition, tropolone produced damaging structural changes seen by both scanning and transmission electron microscopic examination. These changes were both variable and not predictable. Shortening of the incubation time of the chelator with the cell provided the least amount of cellular injury. These findings suggest that tropolone be used as an alternative mediator of lymphocyte labeling with 111In only under critically defined conditions.

Original languageEnglish (US)
Pages (from-to)306-314
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume107
Issue number4
StatePublished - 1986

Fingerprint

Tropolone
Lymphocytes
Oxyquinoline
Labeling
Chelating Agents
Indium Radioisotopes
Pokeweed Mitogens
Lymphokines
Poisons
Wounds and Injuries
Phytohemagglutinins
B-Cell Chronic Lymphocytic Leukemia
Concanavalin A
Toxicity
Microscopic examination
Electrons
Scanning

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Balaban, E. P., Simon, T. R., Sheehan, R. G., & Frenkel, E. P. (1986). Effect of the radiolabel mediator tropolone on lymphocyte structure and function. The Journal of Laboratory and Clinical Medicine, 107(4), 306-314.

Effect of the radiolabel mediator tropolone on lymphocyte structure and function. / Balaban, Edward P.; Simon, Theodore R.; Sheehan, Richard G.; Frenkel, Eugene P.

In: The Journal of Laboratory and Clinical Medicine, Vol. 107, No. 4, 1986, p. 306-314.

Research output: Contribution to journalArticle

Balaban, EP, Simon, TR, Sheehan, RG & Frenkel, EP 1986, 'Effect of the radiolabel mediator tropolone on lymphocyte structure and function', The Journal of Laboratory and Clinical Medicine, vol. 107, no. 4, pp. 306-314.
Balaban, Edward P. ; Simon, Theodore R. ; Sheehan, Richard G. ; Frenkel, Eugene P. / Effect of the radiolabel mediator tropolone on lymphocyte structure and function. In: The Journal of Laboratory and Clinical Medicine. 1986 ; Vol. 107, No. 4. pp. 306-314.
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