Effect of topical hyaluronic acid on experimental cholesteatoma

Steven J. White, Charles G. Wright, Karen S. Robinson, William L. Meyerhoff

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: Inflammation and connective tissue hyperplasia are believed to be important etiological factors in cholesteatoma pathogenesis. Previous work has shown that topically applied hyaluronic acid can reduce connective tissue proliferation in healing wounds and accelerate healing of tympanic membrane perforations. This study was undertaken to determine whether the anti proliferative effect of hyaluronic acid may inhibit propylene glycol-induced cholesteatoma in an animal model. Materials and Methods: A 60% propylene glycol solution was injected bilaterally into the middle ear cavities of 20 adult chinchillas. The control group (N = 10) received propylene glycol alone. In addition to propylene glycol injections, the experimental group (N = 10) received repeated bilateral topical applications of 1.5% hyaluronic acid onto the tympanic membranes. Animals were killed at 4 weeks for gross and light microscopic examination. Results: Seven control and 10 experimental animals survived the full 1-month study period. At the end of that time, cholesteatoma was found in 71% (10/14) of control ears and 70% (14/20) of experimental ears. Tympanic membrane structure did not differ significantly between groups by light microscopy and, in all animals, cholesteatomas originated by migration of hyperplastic epidermis through the tympanic membrane, as has been observed in previous studies using this animal model. Conclusion: Under the conditions of this study, topical hyaluronic acid had no significant effect on cholesteatoma formation.

Original languageEnglish (US)
Pages (from-to)312-318
Number of pages7
JournalAmerican Journal of Otolaryngology--Head and Neck Medicine and Surgery
Volume16
Issue number5
DOIs
StatePublished - 1995

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Cholesteatoma
Hyaluronic Acid
Propylene Glycol
Tympanic Membrane
Connective Tissue
Ear
Animal Models
Tympanic Membrane Perforation
Chinchilla
Light
Middle Ear
Epidermis
Wound Healing
Hyperplasia
Microscopy
Inflammation
Control Groups
Injections

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Effect of topical hyaluronic acid on experimental cholesteatoma. / White, Steven J.; Wright, Charles G.; Robinson, Karen S.; Meyerhoff, William L.

In: American Journal of Otolaryngology--Head and Neck Medicine and Surgery, Vol. 16, No. 5, 1995, p. 312-318.

Research output: Contribution to journalArticle

White, Steven J. ; Wright, Charles G. ; Robinson, Karen S. ; Meyerhoff, William L. / Effect of topical hyaluronic acid on experimental cholesteatoma. In: American Journal of Otolaryngology--Head and Neck Medicine and Surgery. 1995 ; Vol. 16, No. 5. pp. 312-318.
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abstract = "Purpose: Inflammation and connective tissue hyperplasia are believed to be important etiological factors in cholesteatoma pathogenesis. Previous work has shown that topically applied hyaluronic acid can reduce connective tissue proliferation in healing wounds and accelerate healing of tympanic membrane perforations. This study was undertaken to determine whether the anti proliferative effect of hyaluronic acid may inhibit propylene glycol-induced cholesteatoma in an animal model. Materials and Methods: A 60{\%} propylene glycol solution was injected bilaterally into the middle ear cavities of 20 adult chinchillas. The control group (N = 10) received propylene glycol alone. In addition to propylene glycol injections, the experimental group (N = 10) received repeated bilateral topical applications of 1.5{\%} hyaluronic acid onto the tympanic membranes. Animals were killed at 4 weeks for gross and light microscopic examination. Results: Seven control and 10 experimental animals survived the full 1-month study period. At the end of that time, cholesteatoma was found in 71{\%} (10/14) of control ears and 70{\%} (14/20) of experimental ears. Tympanic membrane structure did not differ significantly between groups by light microscopy and, in all animals, cholesteatomas originated by migration of hyperplastic epidermis through the tympanic membrane, as has been observed in previous studies using this animal model. Conclusion: Under the conditions of this study, topical hyaluronic acid had no significant effect on cholesteatoma formation.",
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