Effects of acute and chronic STZ-induced diabetes on clock gene expression and feeding in the gastrointestinal tract

Jonathon Bostwick, Diane Nguyen, Germaine Cornélissen, Franz Halberg, Willemijntje A. Hoogerwerf

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Diabetes may shift clock gene expression within peripheral organs. However, little is known about the effect of diabetes on the gastrointestinal molecular clock. We therefore investigated the effect of diabetes on gastrointestinal clock gene expression. As peripheral clock gene expression is strongly driven by food intake, we also determined the effect of STZ-induced diabetes on patterns of food intake. The effects of acute (1 week) and chronic (12 weeks) STZ-induced diabetes on period (per) genes in the stomach body, proximal and distal colon, liver, kidney, and lung of C57BL/6J mice were assessed using real-time polymerase chain reaction. Food intake studies were completed using automated feeding equipment. Rhythmicity in expression of per2 and per3 persisted in all organs. However, per2 and per3 expression of STZ-injected mice was generally phase delayed within the gastrointestinal tract but not within the kidney or lung as compared with vehicle-injected mice. The phase delay was most pronounced for per2 in the proximal colon at 12 weeks. Food intake was rhythmic with larger circadian amplitude for diabetic mice than for control mice. Thus, STZ-induced diabetes differentially alters peripheral per expression. STZ-induced diabetes does not alter the circadian phase of food intake. Alterations in clock gene expression in a mouse model of diabetes are most pronounced in those organs that are intimately associated with food processing and metabolism.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalMolecular and Cellular Biochemistry
Volume338
Issue number1-2
DOIs
StatePublished - May 1 2010

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Medical problems
Gene expression
Gastrointestinal Tract
Clocks
Eating
Gene Expression
Colon
Kidney
Lung
Food Handling
Periodicity
Inbred C57BL Mouse
Real-Time Polymerase Chain Reaction
Stomach
Food processing
Polymerase chain reaction
Equipment and Supplies
Metabolism
Liver
Genes

Keywords

  • Circadian biology
  • Clock genes
  • Diabetes
  • Gastrointestinal tract
  • Intestine
  • Motility
  • Streptozotocin

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Effects of acute and chronic STZ-induced diabetes on clock gene expression and feeding in the gastrointestinal tract. / Bostwick, Jonathon; Nguyen, Diane; Cornélissen, Germaine; Halberg, Franz; Hoogerwerf, Willemijntje A.

In: Molecular and Cellular Biochemistry, Vol. 338, No. 1-2, 01.05.2010, p. 203-213.

Research output: Contribution to journalArticle

Bostwick, Jonathon ; Nguyen, Diane ; Cornélissen, Germaine ; Halberg, Franz ; Hoogerwerf, Willemijntje A. / Effects of acute and chronic STZ-induced diabetes on clock gene expression and feeding in the gastrointestinal tract. In: Molecular and Cellular Biochemistry. 2010 ; Vol. 338, No. 1-2. pp. 203-213.
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