Effects of Androgen and Estrogen on DNA Synthesis in Immature Rat Prostate

Hideki Kudo, Shinobu Sakamoto, Yoshie Sugiura, Tohru Kawasaki, Tohru Nakayama, Noriyuki Kasahara, Ryohei Okamoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated the effects of testosterone propionate (T) and/or estradiol (E2) on the activity of thymidine kinase (TK) and its isozymes and the incorporation of 3H-thymidine in the prostate of immature rats. The maximum activity of prostatic TK increased approximately 50 times the basal level 48 hr after T administration. Puromycin and actinomycin D inhibited nearly 100% of the increase induced by T administration. The prostatic TK isozymes were separated into 3 types by DEAE-cellulose column chromatography, and the activity of the isozyme eluted with buffer alone was markedly increased in T-treated rats, and this isozyme was suggested to be involved in rapid DNA replication because its activity was not affected by deoxycytidine triphosphate (dCTP). The addition of E2 quickened the induction of maximum TK activity due to T administration. Autoradiographic examination indicated that T alone induced marked DNA synthesis in prostatic epithelium, and E2 plus T enhanced DNA synthesis synergistically.

Original languageEnglish (US)
Pages (from-to)305-313
Number of pages9
JournalActa Histochemica et Cytochemica
Volume20
Issue number3
DOIs
StatePublished - 1987

Fingerprint

Thymidine Kinase
Androgens
Isoenzymes
Rats
Prostate
Estrogens
DNA
Testosterone Propionate
DEAE-Cellulose Chromatography
Puromycin
DEAE-Cellulose
Column chromatography
Dactinomycin
DNA Replication
Thymidine
Estradiol
Buffers
Epithelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

Cite this

Kudo, H., Sakamoto, S., Sugiura, Y., Kawasaki, T., Nakayama, T., Kasahara, N., & Okamoto, R. (1987). Effects of Androgen and Estrogen on DNA Synthesis in Immature Rat Prostate. Acta Histochemica et Cytochemica, 20(3), 305-313. https://doi.org/10.1267/ahc.20.305

Effects of Androgen and Estrogen on DNA Synthesis in Immature Rat Prostate. / Kudo, Hideki; Sakamoto, Shinobu; Sugiura, Yoshie; Kawasaki, Tohru; Nakayama, Tohru; Kasahara, Noriyuki; Okamoto, Ryohei.

In: Acta Histochemica et Cytochemica, Vol. 20, No. 3, 1987, p. 305-313.

Research output: Contribution to journalArticle

Kudo, H, Sakamoto, S, Sugiura, Y, Kawasaki, T, Nakayama, T, Kasahara, N & Okamoto, R 1987, 'Effects of Androgen and Estrogen on DNA Synthesis in Immature Rat Prostate', Acta Histochemica et Cytochemica, vol. 20, no. 3, pp. 305-313. https://doi.org/10.1267/ahc.20.305
Kudo H, Sakamoto S, Sugiura Y, Kawasaki T, Nakayama T, Kasahara N et al. Effects of Androgen and Estrogen on DNA Synthesis in Immature Rat Prostate. Acta Histochemica et Cytochemica. 1987;20(3):305-313. https://doi.org/10.1267/ahc.20.305
Kudo, Hideki ; Sakamoto, Shinobu ; Sugiura, Yoshie ; Kawasaki, Tohru ; Nakayama, Tohru ; Kasahara, Noriyuki ; Okamoto, Ryohei. / Effects of Androgen and Estrogen on DNA Synthesis in Immature Rat Prostate. In: Acta Histochemica et Cytochemica. 1987 ; Vol. 20, No. 3. pp. 305-313.
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