The effects of testosterone and 17β-estradioI on uterine thymidine kinase (TK) activity in immature rats were investigated. Injection of either compound alone resulted in a more than 30-fold increase in TK activity 30 hr later. Injection of the two compounds together resulted in an even greater increase in TK activity and in the activities of its isozymes separated by DEAE cellulose column chromatography. Autoradiographic examination showed that testosterone alone induced marked DNA synthesis in the endometrial stroma and myometrium, but not in the endometrial epithelium, and that its effect on DNA synthesis in all these tissues was enhanced by the additional injection of 17β-estradiol.
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Cell Biology