Effects of anesthesia on functional activation of cerebral blood flow and metabolism

Yasuaki Nakao, Yoshiaki Itoh, Tang Yong Kuang, Michelle Cook, Jane Jehle, Louis Sokoloff

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Functional brain mapping based on changes in local cerebral blood flow (ICBF) or glucose utilization (ICMRglc) induced by functional activation is generally carried out in animals under anesthesia, usually α-chloralose because of its lesser effects on cardiovascular, respiratory, and reflex functions. Results of studies on the role of nitric oxide (NO) in the mechanism of functional activation of ICBF have differed in unanesthetized and anesthetized animals. NO synthase inhibition markedly attenuates or eliminates the ICBF responses in anesthetized animals but not in unanesthetized animals. The present study examines in conscious rats and rats anesthetized with α-chloralose the effects of vibrissal stimulation on ICMRglc and ICBF in the whisker-to-barrel cortex pathway and on the effects of NO synthase inhibition with NG-nitro-L-arginine methyl ester (L-NAME) on the magnitude of the responses. Anesthesia markedly reduced the ICBF and ICMRglc responses in the ventral posteromedial thalamic nucleus and barrel cortex but not in the spinal and principal trigeminal nuclei. L-NAME did not alter the ICBF responses in any of the structures of the pathway in the unanesthetized rats and also not in the trigeminal nuclei of the anesthetized rats. In the thalamus and sensory cortex of the anesthetized rats, where the ICBF responses to stimulation had already been drastically diminished by the anesthesia, L-NAME treatment resulted in loss of statistically significant activation of ICBF by vibrissal stimulation. These results indicate that NO does not mediate functional activation of ICBF under physiological conditions.

Original languageEnglish (US)
Pages (from-to)7593-7598
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number13
DOIs
StatePublished - Jun 19 2001

Fingerprint

Cerebrovascular Circulation
Anesthesia
NG-Nitroarginine Methyl Ester
Chloralose
Nitric Oxide Synthase
Nitric Oxide
Spinal Trigeminal Nucleus
Trigeminal Nuclei
Brain Mapping
Ventral Thalamic Nuclei
Vibrissae
Thalamus
Reflex

Keywords

  • Cerebral glucose utilization
  • Deoxy[C]glucose
  • Functional brain imaging
  • Iodo[C]antipyrine
  • Whisker-to-barrel cortex pathway

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Effects of anesthesia on functional activation of cerebral blood flow and metabolism. / Nakao, Yasuaki; Itoh, Yoshiaki; Kuang, Tang Yong; Cook, Michelle; Jehle, Jane; Sokoloff, Louis.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 13, 19.06.2001, p. 7593-7598.

Research output: Contribution to journalArticle

Nakao, Yasuaki ; Itoh, Yoshiaki ; Kuang, Tang Yong ; Cook, Michelle ; Jehle, Jane ; Sokoloff, Louis. / Effects of anesthesia on functional activation of cerebral blood flow and metabolism. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 13. pp. 7593-7598.
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