Effects of ATP precursors on ATP and free ADP content and functional recovery of postischemic hearts

G. Ambrosio, W. E. Jacobus, M. C. Mitchell, M. R. Litt, L. C. Becker

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

It has been proposed that administration of adenine nucleotide precursors might accelerate replenishment of myocardial ATP and 'free' ADP, thus improving recovery of depressed contractility of postischemic hearts. To test this hypothesis, Langendorff-perfused rabbit hearts were subjected to 20 min of global ischemia and reperfused for 2 h with normal perfusate (n = 8) or perfusate containing 100 μmol/l of the ATP precursors adenosine (n = 8) or 5-amino-4-imidazolecarboxamide riboside (AICAriboside; n = 8). After reperfusion, developed pressure in untreated hearts averaged 70-80% of base line, whereas ATP content was reduced to ~70% of preischemic values. AICAriboside administration did not increase tissue ATP levels or contractility. However, in every heart that received adenosine during reperfusion, ATP content increased from a mean value of 65 ± 4% of base line to 84 ± 5% at the end of reperfusion (P < 0.001). Free ADP also increased in adenosine-treated hearts from 40 to 50% of base line at the beginning of reperfusion, to normal levels by 60 min. However, no improvement in contractility was observed in the hearts that received adenosine. These results support the hypothesis that decreased availability of nucleotide precursors is responsible for depressed ATP levels in postischemic hearts; however, reduced ATP and free ADP levels may not be directly responsible for the depressed function of stunned myocardium.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume256
Issue number2
StatePublished - 1989

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Adenosine Diphosphate
Adenosine Triphosphate
Adenosine
Reperfusion
Myocardial Stunning
Myocardial Contraction
Adenine Nucleotides
Ischemia
Nucleotides
Rabbits
Pressure

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of ATP precursors on ATP and free ADP content and functional recovery of postischemic hearts. / Ambrosio, G.; Jacobus, W. E.; Mitchell, M. C.; Litt, M. R.; Becker, L. C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 256, No. 2, 1989.

Research output: Contribution to journalArticle

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