Abstract
Large cutaneous burns are clearly recognized to produce acute myocardial contractile dysfunction. This study used a model of burn serum challenge in either primary cardiomyocyte cultures or isolated perfused hearts to examine several aspects of burn-serum-related contractile dysfunction as well as myocardial inflammatory responses. Despite the absence of detectable LPS in burn serum, pretreating isolated cells or perfused hearts with recombinant bactericidal permeability-increasing protein (rBPI21) prevented both the inflammatory cytokine cascade and the cardiac contractile dysfunction induced by burn serum treatment of myocytes or ventricular muscle preparations. Our finding that anti-TNF strategies applied to isolated myocytes or hearts before burn serum challenge prevented myocardial inflammation and contractile dysfunction suggested that LPS or LPS-like factors may require the action of second messengers such as TNF-α and IL-1β to mediate LPS-related myocardial depressant effects. Our finding that experimental approaches neutralizing circulating LPS provided cardioprotection suggested that bacterial endotoxin or LPS-like molecules contribute, in part, to burn-related myocardial contractile dysfunction.
Original language | English (US) |
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Pages (from-to) | 438-445 |
Number of pages | 8 |
Journal | Shock |
Volume | 22 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2004 |
Keywords
- Adult rats
- Inflammatory cytokines
- Isolated cardiomyocytes
- Langendorff-perfused hearts
- rBPI
ASJC Scopus subject areas
- Emergency Medicine
- Critical Care and Intensive Care Medicine