Effects of burn serum on myocardial inflammation and function

Jureta W. Horton, David L. Maass, D. Jean White, Billy Sanders, Joseph Murphy

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Large cutaneous burns are clearly recognized to produce acute myocardial contractile dysfunction. This study used a model of burn serum challenge in either primary cardiomyocyte cultures or isolated perfused hearts to examine several aspects of burn-serum-related contractile dysfunction as well as myocardial inflammatory responses. Despite the absence of detectable LPS in burn serum, pretreating isolated cells or perfused hearts with recombinant bactericidal permeability-increasing protein (rBPI21) prevented both the inflammatory cytokine cascade and the cardiac contractile dysfunction induced by burn serum treatment of myocytes or ventricular muscle preparations. Our finding that anti-TNF strategies applied to isolated myocytes or hearts before burn serum challenge prevented myocardial inflammation and contractile dysfunction suggested that LPS or LPS-like factors may require the action of second messengers such as TNF-α and IL-1β to mediate LPS-related myocardial depressant effects. Our finding that experimental approaches neutralizing circulating LPS provided cardioprotection suggested that bacterial endotoxin or LPS-like molecules contribute, in part, to burn-related myocardial contractile dysfunction.

Original languageEnglish (US)
Pages (from-to)438-445
Number of pages8
JournalShock
Volume22
Issue number5
DOIs
StatePublished - Nov 2004

Keywords

  • Adult rats
  • Inflammatory cytokines
  • Isolated cardiomyocytes
  • Langendorff-perfused hearts
  • rBPI

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

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