Effects of chronic ethanol exposure on cardiac receptor-adenylyl cyclase coupling: Studies in cultured embryonic chick myocytes and ethanol fed rats

R. S. Blumenthal, I. W. Flinn, O. Proske, D. G. Jackson, R. G. Tena, M. C. Mitchell, A. M. Feldman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ethanol effects in the brain appear to be mediated at least in part by an alteration in receptor-effector coupling via guanine nucleotide-binding regulatory proteins (G proteins). To test the hypothesis that a similar pathway participates in the cardiotoxic effects of ethanol, we assessed the effects of chronic ethanol on two commonly used experimental models: embryonic chick myocytes in culture and ventricular myocardium from chronically fed rats. Ethanol had no effect on either the function or quantity of G proteins as assessed by effector-stimulated adenylyl cyclase activity and the levels of ADP-ribosylation substrates. In contrast, effector-stimulated adenylyl cyclase activity was significantly altered in the liver of ethanol-fed rats. These results suggest that receptor-effector coupling via G proteins in our two cardiac models is insensitive to ethanol and that ethanol effects may be species or organ specific.

Original languageEnglish (US)
Pages (from-to)1077-1083
Number of pages7
JournalAlcoholism: Clinical and Experimental Research
Volume15
Issue number6
StatePublished - 1991

Fingerprint

Adenylyl Cyclases
Muscle Cells
Rats
Ethanol
Guanine Nucleotides
GTP-Binding Proteins
Carrier Proteins
Proteins
Liver
Adenosine Diphosphate
Brain
Myocardium
Theoretical Models
Substrates

Keywords

  • Adenylyl cyclase
  • Ethanol
  • G protein
  • Myocardial function

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Effects of chronic ethanol exposure on cardiac receptor-adenylyl cyclase coupling : Studies in cultured embryonic chick myocytes and ethanol fed rats. / Blumenthal, R. S.; Flinn, I. W.; Proske, O.; Jackson, D. G.; Tena, R. G.; Mitchell, M. C.; Feldman, A. M.

In: Alcoholism: Clinical and Experimental Research, Vol. 15, No. 6, 1991, p. 1077-1083.

Research output: Contribution to journalArticle

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