Effects of Dibutyryl Cyclic Adenosine Phosphate Plus Theophylline on Murine Sarcoma Virus Transformed Non-Producer Cells

A. Gazdar, M. Hatanaka, R. Herberman, E. Russell, Y. Ikawa

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Treatment of Kirsten sarcoma virus-transformed non-producer cells. (Ki-3T3) with dibutyryl cyclic adenosine phosphate and theophylline (dbcAMP + Th) resulted in marked changes in morphology, reduction in growth rate, and increased adhesiveness, suggesting a reversal towards the non-transformed BALB/3T3 (B-3T3) parent cell line. The morphologic changes were reversible by removal of the dbcAMP + Th. However, treatment of Ki-3T3 cells had little or no effect on the replication of leukemia virus, the presence and rescuability of the sarcoma genome, and the expression of virus-directed cell surface antigens. Sarcoma virus-transformed cells, including Ki-3T3 cells, characteristically have lowered Km values for glucose transport. The treatment did not affect the Km value, indicating that at least one important biochemical marker of the virus transformed state is unaffected by dbcAMP + Th treatment. Treated Ki-3T3 and B-3T3 cells had markedly increased Vmax values for glucose transport.

Original languageEnglish (US)
Pages (from-to)1044-1050
Number of pages7
JournalProceedings of the Society for Experimental Biology and Medicine
Volume141
Issue number3
DOIs
StatePublished - Dec 1972

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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