Effects of exercise training on dendritic morphology in the cardiorespiratory and locomotor centers of the mature rat brain

Amanda J. Nelson, Janice M. Juraska, Brian G. Ragan, Gary A. Iwamoto

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It has been shown that dendritic branching in neural cardiorespiratory and locomotor centers can be attenuated with exercise training (ET) initiated, immediately after weaning. The purpose of this study was to determine whether neuroplastic changes occur within cardiorespiratory and locomotor centers due to ET after maturation. Male SpragueDawley rats (21 days old, n = 28) were individually housed in standard cages. At 91 days of age, animals were divided into two groups: untrained (UN; n = 14) and trained (TR; n = 14). The TR group exercised spontaneously for 50 days on running wheels. ET indexes were obtained, including maximal O2 consumption, percent body fat, resting heart rate, and heart weight-to-body weight ratios. The brain was processed with a modified Golgi-Cox procedure. Impregnated neurons from the periaqueductal gray (PAG), posterior hypothalamic area (PH), nucleus of the tractus solitarius (NTS), cuneiform nucleus (CnF), rostral ventrolateral medulla, nucleus cuneatus, and cerebral cortex were examined. Neurons were traced and analyzed using the Sholl concentric ring analysis of dendritic branching. The mean total number of dendritic intersections with the concentric rings per neuron per animal were compared between. UN and TR groups. There were significant differences between UN and TR groups in the PH, PAG, CnF, and NTS in the total number of intersections per animal. In some areas, the effect size was smaller when ET was initiated in mature animals, possibly related to their relatively reduced activity levels. In conclusion, the adult rat brain remains dynamic and adapts to chronic ET. However, some brain areas appear to be more affected if ET is initiated in early postnatal development.

Original languageEnglish (US)
Pages (from-to)1582-1590
Number of pages9
JournalJournal of Applied Physiology
Volume108
Issue number6
DOIs
StatePublished - Jun 2010

Fingerprint

Exercise
United Nations
Brain
Periaqueductal Gray
Solitary Nucleus
Neurons
Weaning
Running
Cerebral Cortex
Adipose Tissue
Heart Rate
Body Weight
Weights and Measures
Midbrain Reticular Formation

Keywords

  • Adult plasticity
  • Dendritic branching
  • Golgi staining

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Effects of exercise training on dendritic morphology in the cardiorespiratory and locomotor centers of the mature rat brain. / Nelson, Amanda J.; Juraska, Janice M.; Ragan, Brian G.; Iwamoto, Gary A.

In: Journal of Applied Physiology, Vol. 108, No. 6, 06.2010, p. 1582-1590.

Research output: Contribution to journalArticle

@article{8707b498775f425cba8a35f7edbd09b9,
title = "Effects of exercise training on dendritic morphology in the cardiorespiratory and locomotor centers of the mature rat brain",
abstract = "It has been shown that dendritic branching in neural cardiorespiratory and locomotor centers can be attenuated with exercise training (ET) initiated, immediately after weaning. The purpose of this study was to determine whether neuroplastic changes occur within cardiorespiratory and locomotor centers due to ET after maturation. Male SpragueDawley rats (21 days old, n = 28) were individually housed in standard cages. At 91 days of age, animals were divided into two groups: untrained (UN; n = 14) and trained (TR; n = 14). The TR group exercised spontaneously for 50 days on running wheels. ET indexes were obtained, including maximal O2 consumption, percent body fat, resting heart rate, and heart weight-to-body weight ratios. The brain was processed with a modified Golgi-Cox procedure. Impregnated neurons from the periaqueductal gray (PAG), posterior hypothalamic area (PH), nucleus of the tractus solitarius (NTS), cuneiform nucleus (CnF), rostral ventrolateral medulla, nucleus cuneatus, and cerebral cortex were examined. Neurons were traced and analyzed using the Sholl concentric ring analysis of dendritic branching. The mean total number of dendritic intersections with the concentric rings per neuron per animal were compared between. UN and TR groups. There were significant differences between UN and TR groups in the PH, PAG, CnF, and NTS in the total number of intersections per animal. In some areas, the effect size was smaller when ET was initiated in mature animals, possibly related to their relatively reduced activity levels. In conclusion, the adult rat brain remains dynamic and adapts to chronic ET. However, some brain areas appear to be more affected if ET is initiated in early postnatal development.",
keywords = "Adult plasticity, Dendritic branching, Golgi staining",
author = "Nelson, {Amanda J.} and Juraska, {Janice M.} and Ragan, {Brian G.} and Iwamoto, {Gary A.}",
year = "2010",
month = "6",
doi = "10.1152/japplphysiol.00137.2009",
language = "English (US)",
volume = "108",
pages = "1582--1590",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",
number = "6",

}

TY - JOUR

T1 - Effects of exercise training on dendritic morphology in the cardiorespiratory and locomotor centers of the mature rat brain

AU - Nelson, Amanda J.

AU - Juraska, Janice M.

AU - Ragan, Brian G.

AU - Iwamoto, Gary A.

PY - 2010/6

Y1 - 2010/6

N2 - It has been shown that dendritic branching in neural cardiorespiratory and locomotor centers can be attenuated with exercise training (ET) initiated, immediately after weaning. The purpose of this study was to determine whether neuroplastic changes occur within cardiorespiratory and locomotor centers due to ET after maturation. Male SpragueDawley rats (21 days old, n = 28) were individually housed in standard cages. At 91 days of age, animals were divided into two groups: untrained (UN; n = 14) and trained (TR; n = 14). The TR group exercised spontaneously for 50 days on running wheels. ET indexes were obtained, including maximal O2 consumption, percent body fat, resting heart rate, and heart weight-to-body weight ratios. The brain was processed with a modified Golgi-Cox procedure. Impregnated neurons from the periaqueductal gray (PAG), posterior hypothalamic area (PH), nucleus of the tractus solitarius (NTS), cuneiform nucleus (CnF), rostral ventrolateral medulla, nucleus cuneatus, and cerebral cortex were examined. Neurons were traced and analyzed using the Sholl concentric ring analysis of dendritic branching. The mean total number of dendritic intersections with the concentric rings per neuron per animal were compared between. UN and TR groups. There were significant differences between UN and TR groups in the PH, PAG, CnF, and NTS in the total number of intersections per animal. In some areas, the effect size was smaller when ET was initiated in mature animals, possibly related to their relatively reduced activity levels. In conclusion, the adult rat brain remains dynamic and adapts to chronic ET. However, some brain areas appear to be more affected if ET is initiated in early postnatal development.

AB - It has been shown that dendritic branching in neural cardiorespiratory and locomotor centers can be attenuated with exercise training (ET) initiated, immediately after weaning. The purpose of this study was to determine whether neuroplastic changes occur within cardiorespiratory and locomotor centers due to ET after maturation. Male SpragueDawley rats (21 days old, n = 28) were individually housed in standard cages. At 91 days of age, animals were divided into two groups: untrained (UN; n = 14) and trained (TR; n = 14). The TR group exercised spontaneously for 50 days on running wheels. ET indexes were obtained, including maximal O2 consumption, percent body fat, resting heart rate, and heart weight-to-body weight ratios. The brain was processed with a modified Golgi-Cox procedure. Impregnated neurons from the periaqueductal gray (PAG), posterior hypothalamic area (PH), nucleus of the tractus solitarius (NTS), cuneiform nucleus (CnF), rostral ventrolateral medulla, nucleus cuneatus, and cerebral cortex were examined. Neurons were traced and analyzed using the Sholl concentric ring analysis of dendritic branching. The mean total number of dendritic intersections with the concentric rings per neuron per animal were compared between. UN and TR groups. There were significant differences between UN and TR groups in the PH, PAG, CnF, and NTS in the total number of intersections per animal. In some areas, the effect size was smaller when ET was initiated in mature animals, possibly related to their relatively reduced activity levels. In conclusion, the adult rat brain remains dynamic and adapts to chronic ET. However, some brain areas appear to be more affected if ET is initiated in early postnatal development.

KW - Adult plasticity

KW - Dendritic branching

KW - Golgi staining

UR - http://www.scopus.com/inward/record.url?scp=77953174760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953174760&partnerID=8YFLogxK

U2 - 10.1152/japplphysiol.00137.2009

DO - 10.1152/japplphysiol.00137.2009

M3 - Article

C2 - 20339008

AN - SCOPUS:77953174760

VL - 108

SP - 1582

EP - 1590

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

IS - 6

ER -