Effects of glucocorticoid treatment on cardiac protein synthesis and degradation

A. F. Clark, G. N. DeMartino, K. Wildenthal

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We treated rats with dexamethasone (DEX, 1 mg·kg-1·day-1) and examined the effects of this glucocorticoid on heart protein metabolism using atrial explant and Langendorff perfusion preparations. Fasted rats treated with DEX for 2 days had significantly lower body weights (92% of control, P < 0.001) and larger hearts (106% of control, P < 0.005) than fasted control animals. Protein and RNA concentrations remained constant. In atrial explants, DEX treatment produced a 19% increase in protein synthesis (P < 0.001) and a 13% increase in protein degradation (P < 0.002). In Langendorff-perfused hearts, DEX treatment caused a 36% increase in protein synthesis (P < 0.02), while protein degradation was 8% above control (P > 0.05). Thus, in contrast to their catabolic effects on skeletal muscle, glucocorticoids are anabolic on the heart. The increased accumulation of total cardiac protein during early glucocorticoid administration is mediated entirely via increased rates of synthesis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume250
Issue number6
StatePublished - 1986

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Glucocorticoids
Proteolysis
Degradation
Rats
Proteins
Metabolism
Dexamethasone
Muscle
Skeletal Muscle
Perfusion
Body Weight

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Effects of glucocorticoid treatment on cardiac protein synthesis and degradation. / Clark, A. F.; DeMartino, G. N.; Wildenthal, K.

In: American Journal of Physiology - Cell Physiology, Vol. 250, No. 6, 1986.

Research output: Contribution to journalArticle

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