TY - JOUR
T1 - Effects of Helicobacter pylori Infection on the Link between Regenerating Gene Expression and Serum Gastrin Levels in Mongolian Gerbils
AU - Fukui, Hirokazu
AU - Franceschi, Francesco
AU - Penland, Rebecca L.
AU - Sakai, Taro
AU - Sepulveda, Antonia R.
AU - Fujimori, Takahiro
AU - Terano, Akira
AU - Chiba, Tsutomu
AU - Genta, Robert M.
N1 - Funding Information:
This work was supported by the Department of Veterans Affairs, Washington, D.C., and by an unrestricted grant from Otsuka Pharmaceuticals, Osaka, Japan. Dr. Fukui is supported by an educational grant from Otsuka Pharmaceuticals and by the Department of Pathology, Dokkyo University School of Medicine, Tochigi, Japan. Address reprint requests to: Dr. Tsutomu Chiba, Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, 54, Kawaramachi-shogoin, Sakyo-ku, Kyoto 606–8507, Japan. E-mail: cteya@kuhp.kyoto-u.ac.jp
PY - 2003/12
Y1 - 2003/12
N2 - Although regenerating gene (Reg) protein is reported to have atrophic effect on gastric epithelial cells, its involvement in human gastric diseases is not clear. We have recently shown that both gastrin and gastric mucosal inflammation enhance Reg gene expression in the fundic mucosa in rats. This study was designed to clarify whether Reg protein is involved in Helicobacter pylori-induced gastritis and whether Reg gene expression is linked to serum gastrin levels in this condition. Mongolian gerbils were inoculated with an H. pylori strain isolated from a gastric cancer patient. Four weeks later, some of the gerbils with H. pylori infection were eradicated by lansoprazole, amoxicillin, and clarithromycin. The time courses of changes in Reg gene expression, serum gastrin levels, gastric acidity, and histopathologic factors were examined. Four weeks after H. pylori infection, gastritis started spreading to the fundic mucosa, and gastric acidity started reducing. Serum gastrin levels and Reg mRNA expression in the fundus were significantly increased 6 weeks after infection. Reg mRNA expression in the fundus correlated significantly with both serum gastrin levels and the severity of fundic mucosal inflammation. After H. pylori eradication, serum gastrin levels and fundic mucosal inflammation were normalized, and the increase in Reg mRNA expression was abolished. The Reg gene is associated with hypergastrinemia and fundic mucosal inflammation and may be involved in H. pylori-induced gastritis.
AB - Although regenerating gene (Reg) protein is reported to have atrophic effect on gastric epithelial cells, its involvement in human gastric diseases is not clear. We have recently shown that both gastrin and gastric mucosal inflammation enhance Reg gene expression in the fundic mucosa in rats. This study was designed to clarify whether Reg protein is involved in Helicobacter pylori-induced gastritis and whether Reg gene expression is linked to serum gastrin levels in this condition. Mongolian gerbils were inoculated with an H. pylori strain isolated from a gastric cancer patient. Four weeks later, some of the gerbils with H. pylori infection were eradicated by lansoprazole, amoxicillin, and clarithromycin. The time courses of changes in Reg gene expression, serum gastrin levels, gastric acidity, and histopathologic factors were examined. Four weeks after H. pylori infection, gastritis started spreading to the fundic mucosa, and gastric acidity started reducing. Serum gastrin levels and Reg mRNA expression in the fundus were significantly increased 6 weeks after infection. Reg mRNA expression in the fundus correlated significantly with both serum gastrin levels and the severity of fundic mucosal inflammation. After H. pylori eradication, serum gastrin levels and fundic mucosal inflammation were normalized, and the increase in Reg mRNA expression was abolished. The Reg gene is associated with hypergastrinemia and fundic mucosal inflammation and may be involved in H. pylori-induced gastritis.
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U2 - 10.1097/01.LAB.0000106501.56339.CE
DO - 10.1097/01.LAB.0000106501.56339.CE
M3 - Article
C2 - 14691296
AN - SCOPUS:0347995027
SN - 0023-6837
VL - 83
SP - 1777
EP - 1786
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 12
ER -