Effects of histamine on dentate granule cells in vitro

R. W. Greene, H. L. Haas

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Hippocampal slices from rat brain were exposed to histamine and related substances in a perfusion chamber. Granule cells of the dentate gyrus were studied with conventional extra- and intracellular recording and a single electrode voltage clamp. Histamine caused, through activation of H2-receptors, a small depolarization, an increase in the number of synaptic and action potentials, a block of the long lasting (but not the early) component of spike afterhyperpolarizations and a reduction of the accommodation of action potential firing. These effects were mimicked by forskolin (suggests activation of adenylate cyclase). In voltage clamp, histamine blocked a long lasting calcium-dependent outward tail current without any reduction of inward current. Thus histamine selectively blocks the late calcium-dependent potassium current in dentate granule cells which receive histaminergic input from the posterior hypothalamus. Histamine also reduces the field excitatory postsynaptic potential evoked by perforant path stimulation. These actions allow for a powerful modulation of excitatory signals and an effective regulation of hippocampal excitability.

Original languageEnglish (US)
Pages (from-to)299-303
Number of pages5
JournalNeuroscience
Volume34
Issue number2
DOIs
StatePublished - 1990

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Histamine
Action Potentials
Posterior Hypothalamus
Perforant Pathway
Calcium
Synaptic Potentials
Histamine H2 Receptors
Excitatory Postsynaptic Potentials
Dentate Gyrus
Colforsin
Adenylyl Cyclases
Tail
Potassium
Electrodes
Perfusion
In Vitro Techniques
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effects of histamine on dentate granule cells in vitro. / Greene, R. W.; Haas, H. L.

In: Neuroscience, Vol. 34, No. 2, 1990, p. 299-303.

Research output: Contribution to journalArticle

Greene, R. W. ; Haas, H. L. / Effects of histamine on dentate granule cells in vitro. In: Neuroscience. 1990 ; Vol. 34, No. 2. pp. 299-303.
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