Effects of hypoglycemia and prolonged fasting on insulin and glucagon gene expression. Studies with in situ hybridization

L. Chen, I. Komiya, L. Inman, J. O'Neil, M. Appel, T. Alam, Roger H Unger

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In situ hybridization of proinsulin and proglucagon mRNA was performed in rat pancreas to assess prohormone gene expression during various glucopenic conditions. During a 4-d fast mean blood glucose declined by 48 mg/dl; proinsulin mRNA signal density remained normal while proglucagon mRNA signal density more than doubled. At the end of a continuous 12-d insulin infusion blood glucose averaged 53 ± 12 mg/dl; proinsulin mRNA signal density declined to 30% of controls while proglucagon mRNA signal density more than doubled. In insulinoma-bearing NEDH rats blood glucose averaged 34 ± 3.5 mg/dl; the proinsulin mRNA signal was virtually undetectable and proglucagon mRNA signal density was more than twice the controls. There was no detectable change in either β-cell area or islet number in rats subjected to fasting or insulin infusion, but in insulinoma-bearing rats β cell area was markedly reduced. Thus compensation during 4 d of starvation involves an increase in glucagon gene expression without change in insulin gene expression or β cell mass. In moderate insulin-induced hypoglycemia glucagon gene expression is increased and insulin gene expression decreased. In more profound insulinoma-induced hypoglycemia, in addition to the foregoing changes in hormone gene expression, there is a profound reduction in the number of insulin-expressing cells.

Original languageEnglish (US)
Pages (from-to)711-714
Number of pages4
JournalJournal of Clinical Investigation
Volume84
Issue number2
StatePublished - 1989

Fingerprint

Glucagon
Hypoglycemia
In Situ Hybridization
Proglucagon
Proinsulin
Insulin
Gene Expression
Messenger RNA
Insulinoma
Blood Glucose
Starvation
Pancreas
Fasting
Hormones

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of hypoglycemia and prolonged fasting on insulin and glucagon gene expression. Studies with in situ hybridization. / Chen, L.; Komiya, I.; Inman, L.; O'Neil, J.; Appel, M.; Alam, T.; Unger, Roger H.

In: Journal of Clinical Investigation, Vol. 84, No. 2, 1989, p. 711-714.

Research output: Contribution to journalArticle

Chen, L. ; Komiya, I. ; Inman, L. ; O'Neil, J. ; Appel, M. ; Alam, T. ; Unger, Roger H. / Effects of hypoglycemia and prolonged fasting on insulin and glucagon gene expression. Studies with in situ hybridization. In: Journal of Clinical Investigation. 1989 ; Vol. 84, No. 2. pp. 711-714.
@article{1beb497157714069bcf03dea0c1acce6,
title = "Effects of hypoglycemia and prolonged fasting on insulin and glucagon gene expression. Studies with in situ hybridization",
abstract = "In situ hybridization of proinsulin and proglucagon mRNA was performed in rat pancreas to assess prohormone gene expression during various glucopenic conditions. During a 4-d fast mean blood glucose declined by 48 mg/dl; proinsulin mRNA signal density remained normal while proglucagon mRNA signal density more than doubled. At the end of a continuous 12-d insulin infusion blood glucose averaged 53 ± 12 mg/dl; proinsulin mRNA signal density declined to 30{\%} of controls while proglucagon mRNA signal density more than doubled. In insulinoma-bearing NEDH rats blood glucose averaged 34 ± 3.5 mg/dl; the proinsulin mRNA signal was virtually undetectable and proglucagon mRNA signal density was more than twice the controls. There was no detectable change in either β-cell area or islet number in rats subjected to fasting or insulin infusion, but in insulinoma-bearing rats β cell area was markedly reduced. Thus compensation during 4 d of starvation involves an increase in glucagon gene expression without change in insulin gene expression or β cell mass. In moderate insulin-induced hypoglycemia glucagon gene expression is increased and insulin gene expression decreased. In more profound insulinoma-induced hypoglycemia, in addition to the foregoing changes in hormone gene expression, there is a profound reduction in the number of insulin-expressing cells.",
author = "L. Chen and I. Komiya and L. Inman and J. O'Neil and M. Appel and T. Alam and Unger, {Roger H}",
year = "1989",
language = "English (US)",
volume = "84",
pages = "711--714",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "2",

}

TY - JOUR

T1 - Effects of hypoglycemia and prolonged fasting on insulin and glucagon gene expression. Studies with in situ hybridization

AU - Chen, L.

AU - Komiya, I.

AU - Inman, L.

AU - O'Neil, J.

AU - Appel, M.

AU - Alam, T.

AU - Unger, Roger H

PY - 1989

Y1 - 1989

N2 - In situ hybridization of proinsulin and proglucagon mRNA was performed in rat pancreas to assess prohormone gene expression during various glucopenic conditions. During a 4-d fast mean blood glucose declined by 48 mg/dl; proinsulin mRNA signal density remained normal while proglucagon mRNA signal density more than doubled. At the end of a continuous 12-d insulin infusion blood glucose averaged 53 ± 12 mg/dl; proinsulin mRNA signal density declined to 30% of controls while proglucagon mRNA signal density more than doubled. In insulinoma-bearing NEDH rats blood glucose averaged 34 ± 3.5 mg/dl; the proinsulin mRNA signal was virtually undetectable and proglucagon mRNA signal density was more than twice the controls. There was no detectable change in either β-cell area or islet number in rats subjected to fasting or insulin infusion, but in insulinoma-bearing rats β cell area was markedly reduced. Thus compensation during 4 d of starvation involves an increase in glucagon gene expression without change in insulin gene expression or β cell mass. In moderate insulin-induced hypoglycemia glucagon gene expression is increased and insulin gene expression decreased. In more profound insulinoma-induced hypoglycemia, in addition to the foregoing changes in hormone gene expression, there is a profound reduction in the number of insulin-expressing cells.

AB - In situ hybridization of proinsulin and proglucagon mRNA was performed in rat pancreas to assess prohormone gene expression during various glucopenic conditions. During a 4-d fast mean blood glucose declined by 48 mg/dl; proinsulin mRNA signal density remained normal while proglucagon mRNA signal density more than doubled. At the end of a continuous 12-d insulin infusion blood glucose averaged 53 ± 12 mg/dl; proinsulin mRNA signal density declined to 30% of controls while proglucagon mRNA signal density more than doubled. In insulinoma-bearing NEDH rats blood glucose averaged 34 ± 3.5 mg/dl; the proinsulin mRNA signal was virtually undetectable and proglucagon mRNA signal density was more than twice the controls. There was no detectable change in either β-cell area or islet number in rats subjected to fasting or insulin infusion, but in insulinoma-bearing rats β cell area was markedly reduced. Thus compensation during 4 d of starvation involves an increase in glucagon gene expression without change in insulin gene expression or β cell mass. In moderate insulin-induced hypoglycemia glucagon gene expression is increased and insulin gene expression decreased. In more profound insulinoma-induced hypoglycemia, in addition to the foregoing changes in hormone gene expression, there is a profound reduction in the number of insulin-expressing cells.

UR - http://www.scopus.com/inward/record.url?scp=0024410984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024410984&partnerID=8YFLogxK

M3 - Article

C2 - 2760207

AN - SCOPUS:0024410984

VL - 84

SP - 711

EP - 714

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 2

ER -