Effects of insulin on ribonucleic acid synthesis in toad urinary bladder

M. H. Cobb, C. P H Yang, W. N. Scott

Research output: Contribution to journalArticle

3 Scopus citations


We previously found that the sustained rise in active sodium transport by the toad urinary bladder after exposure to insulin is associated with the synthesis of a membrane protein with a molecular weight of 25,000. Studying the effects of insulin on the incorporation of uridine into RNA of epithelial cells of the bladder, we now report that insulin causes a 39% increase in the incorporation of [3H]uridine into poly(A)-containing RNA extracted from granular cells, the predominant epithelial cell type. The increased accumulation of label in RNA, most notably species ranging from 5-10S, could be demonstrated whether cells were pulsed with [3H]uridine or continuously exposed to the label. Using a reticulocyte lysate system, we compared the translation products of RNA from untreated and insulin-treated granular mucosal cells. Exposure to insulin was associated with the expression of mRNA coding for a protein with molecular weight of approximately 25,000, similar in size to the insulin-induced membrane protein we previously identified in the intact tissue.

Original languageEnglish (US)
Pages (from-to)2167-2174
Number of pages8
Issue number6
Publication statusPublished - 1981


ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Cobb, M. H., Yang, C. P. H., & Scott, W. N. (1981). Effects of insulin on ribonucleic acid synthesis in toad urinary bladder. Endocrinology, 109(6), 2167-2174.