Effects of nicotine on the strength of attachment of gingival fibroblasts to glass and non-diseased human root surfaces

Eduardo Tanur, Michael J. McQuade, James C. McPherson, Ibtisam H. Al-Hashimi, Francisco Rivera-Hidalgo

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: The aim of this study was to evaluate the effect of nicotine on the strength of attachment of human gingival fibroblast cells to glass and non-diseased human root surfaces. Methods: Human gingival fibroblast cells (HGF) were trypsinized, suspended in RPM1 1640 medium, and incubated with autoclaved human root and glass sections and nicotine (NIC) concentrations of 0 (control), 25, 50, and 100 ng/ml for 1 week. HGF attached and grew on glass and root surfaces for 4 weeks at all NIC concentrations. HGF cultures were subjected to a rotary shaker machine for 30 minutes to test the strength of attachment of these cells at 100, 150, and 200 rpm. The root and glass sections were examined at 48 hours by light microscopy. Results: Control groups exhibited a monolayer of long, spindle-shaped fibroblasts with a parallel alignment and minimal overlapping. With a concentration of NIC of 50 or 100 ng/ml as well as with increasing 'speeds,' the number of cells attached to these surfaces decreased dramatically. When 200 rpm was used for both groups at all NIC concentrations, very few HGF remained attached to these surfaces. Conclusions: This study showed that the nature of cell attachment to either glass or root surfaces is altered by nicotine, and marked detachment was noted when nicotine exposure was coupled with vigorous agitation at different rpm. Marked detachment noted in all specimens at 200 rpm indicates that this speed is excessive for use in subsequent experimentation.

Original languageEnglish (US)
Pages (from-to)717-722
Number of pages6
JournalJournal of Periodontology
Volume71
Issue number5
DOIs
StatePublished - Jan 1 2000

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Nicotine
Glass
Fibroblasts
Microscopy
Cell Culture Techniques
Cell Count
Light
Control Groups

Keywords

  • Cell attachment
  • Fibroblasts
  • Glass
  • Nicotine
  • Tooth root

ASJC Scopus subject areas

  • Periodontics

Cite this

Effects of nicotine on the strength of attachment of gingival fibroblasts to glass and non-diseased human root surfaces. / Tanur, Eduardo; McQuade, Michael J.; McPherson, James C.; Al-Hashimi, Ibtisam H.; Rivera-Hidalgo, Francisco.

In: Journal of Periodontology, Vol. 71, No. 5, 01.01.2000, p. 717-722.

Research output: Contribution to journalArticle

Tanur, Eduardo ; McQuade, Michael J. ; McPherson, James C. ; Al-Hashimi, Ibtisam H. ; Rivera-Hidalgo, Francisco. / Effects of nicotine on the strength of attachment of gingival fibroblasts to glass and non-diseased human root surfaces. In: Journal of Periodontology. 2000 ; Vol. 71, No. 5. pp. 717-722.
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