TY - JOUR
T1 - Effects of nucleic acids and polyanions on dimer formation and DNA binding by bZIP and bHLHZip transcription factors
AU - Kohler, Jennifer J.
AU - Schepartz, Alanna
N1 - Funding Information:
This work was supported by the NIH (GM 52544). J.J.K. was supported by a NSF graduate research fellowship and by a predoctoral fellowship provided by Wyeth-Ayerst Research through the American Chemical Society, Division of Medicinal Chemistry. We thank Tanya Schneider for the clone encoding A71, Lin Chen and Steven Harrison for supplying the clones used to generate Fos and Jun expression vectors, and Satish Nair and Stephen Burley for the clones encoding Max 22–113 and Max 22–105 SH . We thank Stacey Rutledge for comments on the manuscript. Fluorescence polarization data was acquired in the laboratory of Paul Sigler.
PY - 2001
Y1 - 2001
N2 - A large fraction of known transcription factors form 2:1 complexes with DNA. In our studies of the assembly of such ternary (protein protein DNA) complexes formed by bZIP an bHLHZip proteins, we foun that the proteins recognize DNA as monomers. Here we show that protein monomer-DNA complexes are favored at high DNA concentrations. Further, we show that, due to fast rates of association with protein monomers, DNA and other polyanions accelerate the rate of protein dimer formation. Finally, we find that DNA-assisted formation of protein dimers provides a mechanism by which dimeric transcription factors can rapidly discriminate between specific and nonspecific sites.
AB - A large fraction of known transcription factors form 2:1 complexes with DNA. In our studies of the assembly of such ternary (protein protein DNA) complexes formed by bZIP an bHLHZip proteins, we foun that the proteins recognize DNA as monomers. Here we show that protein monomer-DNA complexes are favored at high DNA concentrations. Further, we show that, due to fast rates of association with protein monomers, DNA and other polyanions accelerate the rate of protein dimer formation. Finally, we find that DNA-assisted formation of protein dimers provides a mechanism by which dimeric transcription factors can rapidly discriminate between specific and nonspecific sites.
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U2 - 10.1016/S0968-0896(01)00221-8
DO - 10.1016/S0968-0896(01)00221-8
M3 - Article
C2 - 11553485
AN - SCOPUS:0034824698
SN - 0968-0896
VL - 9
SP - 2435
EP - 2443
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 9
ER -