Effects of orchiopexy on congenitally cryptorchid insulin-3 knockout mice

Michael T. Nguyen, Patrick R. Showalter, Charles F. Timmons, Serge Nef, Luis F. Parada, Linda A. Baker, Faruk Hadziselimovic

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Purpose: Insulin-3 (Insl3) knockout mice exhibit isolated, bilateral, high intra-abdominal cryptorchidism. If left in this position until adulthood, these testes will deteriorate to the Sertoli-cell-only state, leading to infertility in 100%. We examined the effect of orchiopexy in this genetically engineered animal model. Materials and Methods: A total of 160 testes from 80 male offspring of Insl3 F1 crosses underwent either no orchiopexy (29 testes), sham surgery (25 testes), 1-stage Fowler-Stephens scrotal orchiopexy (57 testes) or primary orchiopexy into a low abdominal, subcutaneous pouch (49 testes). A group of postoperative mice underwent fertility testing 3 months postoperatively. At 6 to 9 months postoperatively the testes were harvested and histologically analyzed. Results: Testes were atrophic in 100% (25 of 25) of the sham group, 91% (52 of 57) of the Fowler-Stephens orchiopexy group and 33% (16 of 49) of the subcutaneous pouch group (testis weight less than 50 mg.). Fertility testing was done in 30 mice (sham 5, Fowler-Stephens orchiopexy 17 and subcutaneous pouch 8). Infertility was secondary to bilateral testicular atrophy (sham 5 of 5, Fowler-Stephens orchiopexy 13 of 17), spermatogenic maturation arrest (subcutaneous pouch 8 of 8) and ductal obstruction [normal spermatogenesis with epididymis devoid of sperm] (Fowler-Stephens orchiopexy 2 of 17). Fertility with normal spermatogenesis was observed in 2 of 17 Fowler-Stephens orchiopexy mice, both of which were Insl3 knockout mice. Conclusions: Intrascrotal orchiopexy can rescue these congenitally cryptorchid Insl3 knockout testes from their intra-abdominal fate of Sertoli-cell-only and lead to fertility. The results suggest that orchiopexy has a crucial and central role in preservation of spermatogenesis.

Original languageEnglish (US)
Pages (from-to)1779-1783
Number of pages5
JournalJournal of Urology
Volume168
Issue number4 II
StatePublished - Oct 2002

Fingerprint

Orchiopexy
Knockout Mice
Testis
Insulin
Fertility
Spermatogenesis
Sertoli Cells
Infertility
Genetically Modified Animals
Cryptorchidism
Epididymis
Atrophy
Spermatozoa
Animal Models

Keywords

  • Cryptorchidism
  • Fertility, testis
  • Models, animal

ASJC Scopus subject areas

  • Urology

Cite this

Nguyen, M. T., Showalter, P. R., Timmons, C. F., Nef, S., Parada, L. F., Baker, L. A., & Hadziselimovic, F. (2002). Effects of orchiopexy on congenitally cryptorchid insulin-3 knockout mice. Journal of Urology, 168(4 II), 1779-1783.

Effects of orchiopexy on congenitally cryptorchid insulin-3 knockout mice. / Nguyen, Michael T.; Showalter, Patrick R.; Timmons, Charles F.; Nef, Serge; Parada, Luis F.; Baker, Linda A.; Hadziselimovic, Faruk.

In: Journal of Urology, Vol. 168, No. 4 II, 10.2002, p. 1779-1783.

Research output: Contribution to journalArticle

Nguyen, MT, Showalter, PR, Timmons, CF, Nef, S, Parada, LF, Baker, LA & Hadziselimovic, F 2002, 'Effects of orchiopexy on congenitally cryptorchid insulin-3 knockout mice', Journal of Urology, vol. 168, no. 4 II, pp. 1779-1783.
Nguyen MT, Showalter PR, Timmons CF, Nef S, Parada LF, Baker LA et al. Effects of orchiopexy on congenitally cryptorchid insulin-3 knockout mice. Journal of Urology. 2002 Oct;168(4 II):1779-1783.
Nguyen, Michael T. ; Showalter, Patrick R. ; Timmons, Charles F. ; Nef, Serge ; Parada, Luis F. ; Baker, Linda A. ; Hadziselimovic, Faruk. / Effects of orchiopexy on congenitally cryptorchid insulin-3 knockout mice. In: Journal of Urology. 2002 ; Vol. 168, No. 4 II. pp. 1779-1783.
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abstract = "Purpose: Insulin-3 (Insl3) knockout mice exhibit isolated, bilateral, high intra-abdominal cryptorchidism. If left in this position until adulthood, these testes will deteriorate to the Sertoli-cell-only state, leading to infertility in 100{\%}. We examined the effect of orchiopexy in this genetically engineered animal model. Materials and Methods: A total of 160 testes from 80 male offspring of Insl3 F1 crosses underwent either no orchiopexy (29 testes), sham surgery (25 testes), 1-stage Fowler-Stephens scrotal orchiopexy (57 testes) or primary orchiopexy into a low abdominal, subcutaneous pouch (49 testes). A group of postoperative mice underwent fertility testing 3 months postoperatively. At 6 to 9 months postoperatively the testes were harvested and histologically analyzed. Results: Testes were atrophic in 100{\%} (25 of 25) of the sham group, 91{\%} (52 of 57) of the Fowler-Stephens orchiopexy group and 33{\%} (16 of 49) of the subcutaneous pouch group (testis weight less than 50 mg.). Fertility testing was done in 30 mice (sham 5, Fowler-Stephens orchiopexy 17 and subcutaneous pouch 8). Infertility was secondary to bilateral testicular atrophy (sham 5 of 5, Fowler-Stephens orchiopexy 13 of 17), spermatogenic maturation arrest (subcutaneous pouch 8 of 8) and ductal obstruction [normal spermatogenesis with epididymis devoid of sperm] (Fowler-Stephens orchiopexy 2 of 17). Fertility with normal spermatogenesis was observed in 2 of 17 Fowler-Stephens orchiopexy mice, both of which were Insl3 knockout mice. Conclusions: Intrascrotal orchiopexy can rescue these congenitally cryptorchid Insl3 knockout testes from their intra-abdominal fate of Sertoli-cell-only and lead to fertility. The results suggest that orchiopexy has a crucial and central role in preservation of spermatogenesis.",
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AU - Nguyen, Michael T.

AU - Showalter, Patrick R.

AU - Timmons, Charles F.

AU - Nef, Serge

AU - Parada, Luis F.

AU - Baker, Linda A.

AU - Hadziselimovic, Faruk

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N2 - Purpose: Insulin-3 (Insl3) knockout mice exhibit isolated, bilateral, high intra-abdominal cryptorchidism. If left in this position until adulthood, these testes will deteriorate to the Sertoli-cell-only state, leading to infertility in 100%. We examined the effect of orchiopexy in this genetically engineered animal model. Materials and Methods: A total of 160 testes from 80 male offspring of Insl3 F1 crosses underwent either no orchiopexy (29 testes), sham surgery (25 testes), 1-stage Fowler-Stephens scrotal orchiopexy (57 testes) or primary orchiopexy into a low abdominal, subcutaneous pouch (49 testes). A group of postoperative mice underwent fertility testing 3 months postoperatively. At 6 to 9 months postoperatively the testes were harvested and histologically analyzed. Results: Testes were atrophic in 100% (25 of 25) of the sham group, 91% (52 of 57) of the Fowler-Stephens orchiopexy group and 33% (16 of 49) of the subcutaneous pouch group (testis weight less than 50 mg.). Fertility testing was done in 30 mice (sham 5, Fowler-Stephens orchiopexy 17 and subcutaneous pouch 8). Infertility was secondary to bilateral testicular atrophy (sham 5 of 5, Fowler-Stephens orchiopexy 13 of 17), spermatogenic maturation arrest (subcutaneous pouch 8 of 8) and ductal obstruction [normal spermatogenesis with epididymis devoid of sperm] (Fowler-Stephens orchiopexy 2 of 17). Fertility with normal spermatogenesis was observed in 2 of 17 Fowler-Stephens orchiopexy mice, both of which were Insl3 knockout mice. Conclusions: Intrascrotal orchiopexy can rescue these congenitally cryptorchid Insl3 knockout testes from their intra-abdominal fate of Sertoli-cell-only and lead to fertility. The results suggest that orchiopexy has a crucial and central role in preservation of spermatogenesis.

AB - Purpose: Insulin-3 (Insl3) knockout mice exhibit isolated, bilateral, high intra-abdominal cryptorchidism. If left in this position until adulthood, these testes will deteriorate to the Sertoli-cell-only state, leading to infertility in 100%. We examined the effect of orchiopexy in this genetically engineered animal model. Materials and Methods: A total of 160 testes from 80 male offspring of Insl3 F1 crosses underwent either no orchiopexy (29 testes), sham surgery (25 testes), 1-stage Fowler-Stephens scrotal orchiopexy (57 testes) or primary orchiopexy into a low abdominal, subcutaneous pouch (49 testes). A group of postoperative mice underwent fertility testing 3 months postoperatively. At 6 to 9 months postoperatively the testes were harvested and histologically analyzed. Results: Testes were atrophic in 100% (25 of 25) of the sham group, 91% (52 of 57) of the Fowler-Stephens orchiopexy group and 33% (16 of 49) of the subcutaneous pouch group (testis weight less than 50 mg.). Fertility testing was done in 30 mice (sham 5, Fowler-Stephens orchiopexy 17 and subcutaneous pouch 8). Infertility was secondary to bilateral testicular atrophy (sham 5 of 5, Fowler-Stephens orchiopexy 13 of 17), spermatogenic maturation arrest (subcutaneous pouch 8 of 8) and ductal obstruction [normal spermatogenesis with epididymis devoid of sperm] (Fowler-Stephens orchiopexy 2 of 17). Fertility with normal spermatogenesis was observed in 2 of 17 Fowler-Stephens orchiopexy mice, both of which were Insl3 knockout mice. Conclusions: Intrascrotal orchiopexy can rescue these congenitally cryptorchid Insl3 knockout testes from their intra-abdominal fate of Sertoli-cell-only and lead to fertility. The results suggest that orchiopexy has a crucial and central role in preservation of spermatogenesis.

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