Effects of passive immunization of growth hormone-releasing hormone and somatostatin on growth hormone secretion under conditions of high somatostatin tone

Bethany A. Janowski, William B. Wehrenberg

Research output: Contribution to journalArticle

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Abstract

Pulsatile GH secretion decreases during food-deprivation in the rat. It has been hypothesized that this decrease is due to elevated hypothalamic somatostatin secretion. This is based on the observation that GH increases in food-deprived rats following removal of endogenous somatostatin using passive immunization techniques. Cognizant of the important stimulatory effects of growth hormone-releasing hormone (GHRH) on GH secretion, we sought to determine if this neuropeptide plays any role in mediating GH secretion in food-deprived rats. Male rats were prepared with indwelling venous catheters using sodium pentobarbital anesthesia seven days prior to experimentation. Animals were food-deprived for 72 h, after which control blood samples were drawn from -60 to 0 min. One group was then treated with normal rabbit serum (NRS), while a second group was treated with GHRH antiserum (GHRHab). At 55 min all animals received somatostatin antiserum (SSab). No animal exhibited any spontaneous GH peak during the one hour control period or in the subsequent one hour period following the administration of GHRHab or NRS. Absence of GH pulsatility during food-deprivation, coupled with no decrease in GH levels in food-deprived rats treated with GHRHab suggest that diminished GHRH pulsatility is likely during food-deprivation. Subsequent treatment of these animals with SSab resulted in an identical 2.5 fold increase in GH concentrations. This result suggests that GHRH is not involved in the GH rebound following somatostatin withdrawal in food-deprived rats.

Original languageEnglish (US)
Pages (from-to)951-958
Number of pages8
JournalLife Sciences
Volume50
Issue number13
DOIs
StatePublished - 1992

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Immunization
Growth Hormone-Releasing Hormone
Passive Immunization
Somatostatin
Growth Hormone
Rats
Food Deprivation
Food
Animals
Immune Sera
Rabbits
Indwelling Catheters
Pentobarbital
Neuropeptides
Serum
Catheters
Anesthesia
Blood

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of passive immunization of growth hormone-releasing hormone and somatostatin on growth hormone secretion under conditions of high somatostatin tone. / Janowski, Bethany A.; Wehrenberg, William B.

In: Life Sciences, Vol. 50, No. 13, 1992, p. 951-958.

Research output: Contribution to journalArticle

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