Effects of prolonged hypoxia on adenylate cyclase activity and β-adrenergic receptors in pulmonary and systemic arteries of the rat

P. W. Shaul, K. H. Muntz, D. DeBeltz, L. M. Buja

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Prolonged hypoxia causes pulmonary hypertension but no change in systemic vasomotor tone. In an effort to define the mechanisms involved, we determined the effects of 3 and 7 days of hypoxia on adenylate cyclase activity and β-adrenergic receptor binding characteristics in pulmonary and systemic arteries in an adult rat model of hypoxic pulmonary vasoconstriction produced by hypobaria. Basal and stimulated adenylate cyclase activity were measured in crude membrane preparations by radioimmunoassay for cyclic AMP. Basal enzyme activity in pulmonary arteries did not change with hypoxia, whereas in systemic arteries it increased 3.5- and 5.3-fold following 3 and 7 days of hypoxia, respectively. GTP-stimulated activity in pulmonary arteries also did not change, but in systemic arteries it increased 7.1- and 5.5-fold. Isoproterenol-stimulated activity in pulmonary arteries was decreased to 49% of control-stimulated activity following 3 days but was similar to control-stimulated activity after 7 days of hypoxia; in systemic arteries it increased 5.6- and 4.6-fold. Sodium fluoride-stimulated activity in pulmonary arteries was unchanged, whereas in systemic arteries it increased 3.8- and 5.3-fold. In contrast, forskolin-stimulated activity, which also was not altered in pulmonary arteries, was increased by only 85% and 71% in systemic arteries. β-Adrenergic receptors were studied with [125I]iodocyanopindolol. Seven days of hypoxia decreased receptor density by 37% and 57% in the pulmonary and systemic arteries, respectively. Receptor affinity for agonists was not altered. Thus, despite downregulation of β-adrenergic receptors in both artery types, prolonged hypoxia has no sustained effect on adenylate cyclase activity in pulmonary arteries, whereas enzyme activity in systemic arteries is markedly increased. The degrees of enhancement observed with hypoxia in systemic arteries with stimulation at the various points along the cyclase pathway suggest that the augmented enzyme activity is mediated by changes at the level of the stimulatory guanine nucleotide-dependent regulatory protein.

Original languageEnglish (US)
Pages (from-to)1526-1534
Number of pages9
JournalCirculation research
Volume66
Issue number6
DOIs
StatePublished - Jan 1 1990

Keywords

  • G proteins
  • adenylate cyclase
  • aorta
  • hypoxia
  • pulmonary arteries
  • β-adrenergic receptors

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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