Effects of protein kinase C activation on sodium, potassium, chloride, and total CO2 transport in the rabbit cortical collecting tubule

S. R. Hays, M. Baum, J. P. Kokko

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Several hormones induce phosphatidylinositol turnover in cell membranes and thus activate protein kinase C. Activation of protein kinase C can, in turn, have effects on epithelial transport. These experiments were designed to investigate the effects of two activators of protein kinase C, phorbol 12-myristate, 13-acetate (PMA) and L-α-1,2-dioctanoylglycerol (L-α-1,2-DOG), and two inactive analogues, 4α-phorbol and 4-O-methyl phorbol 12-myristate,13-acetate, on sodium, potassium, chloride, and total CO2 transport in the rabbit cortical collecting tubule. Utilizing in vitro microperfusion techniques, we found that activation of protein kinase C with either PMA or L-α-1,2-DOG significantly inhibited net sodium absorption, net potassium secretion and transepithelial voltage in a dose-dependent manner. There was no effect on net chloride or total CO2 transport. In contrast, the inactive phorbol analogues did not alter either sodium or potassium transport. These studies demonstrate that in the rabbit cortical collecting tubule sodium and potassium transport can be inhibited by compounds known to activate protein kinase C. Thus, hormones that induce phosphatidylinositol turnover in the rabbit cortical collecting tubule may lead to inhibition of sodium transport by activation of protein kinase C.

Original languageEnglish (US)
Pages (from-to)1561-1570
Number of pages10
JournalJournal of Clinical Investigation
Volume80
Issue number6
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Medicine(all)

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