TY - JOUR
T1 - Effects of rigid and soft contact lens daily wear on corneal epithelium, tear lactate dehydrogenase, and bacterial binding to exfoliated epithelial cells
AU - Ladage, Patrick M.
AU - Yamamoto, Kazuaki
AU - Ren, David H.
AU - Li, Ling
AU - Jester, James V.
AU - Petroll, Walter M
AU - Cavanagh, Harrison D
N1 - Funding Information:
Supported in part by the National Eye Institute, Bethesda, Maryland (grant no.: EY10738 [HDC]); Bausch and Lomb, Inc., Rochester, New York; Menicon Ltd., Nagoya, Japan; The Pearle Vision and Chilton Foundations, Dallas, Texas; Senior Scientist Awards (JVJ, HDC), Olga Keith Weiss Scholar Award (WMP), and an unrestricted grant from Research to Prevent Blindness, Inc., New York, New York.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Objective: To determine the effects of lens type and oxygen transmissibility on human corneal epithelium during daily lens wear (DW). Design: Prospective, randomized, double-masked, single-center, parallel treatment groups clinical trial. Participants: Two hundred forty-six patients fitted with: (1) high oxygen-transmissible soft lenses (n = 36), (2) hyper oxygen-transmissible soft lenses (n = 135), and (3) hyper oxygen-transmissible rigid gas-permeable (RGP) lenses (n = 75). Intervention: Irrigation chamber to collect exfoliated epithelial surface cells, confocal microscopy, and tear collection at baseline, 2 weeks, and 4 weeks of DW. Main Outcome Measures: (1) Pseudomonas aeruginosa (PA) binding to exfoliated corneal epithelial surface cells, (2) central epithelial thickness, (3) superficial epithelial cell area, (4) epithelial surface cell exfoliation, and (5) tear lactate dehydrogenase (LDH). Results: Four weeks of DW with the high oxygen-transmissible soft lens significantly increased PA binding from baseline 6.55 ± 3.01 to 8.75 ± 3.05 bacteria per epithelial cell (P < 0.01). By contrast, hyper oxygen-transmissible soft lens wear increased binding significantly less (6.13 ± 2.45 to 7.62 ± 3.06; P < 0.01), whereas hyper oxygen-transmissible RGP lens wear demonstrated no significant changes (5.91 ± 2.40 to 6.13 ± 2.17; P = 0.533). No significant change in central epithelial thickness was found after 4 weeks of DW in either soft lens; however, the epithelial thickness decreased by 9.8% (P < 0.001) with RGP lens wear. Epithelial cell surface area increased 3.3% and 4.1% with the high and hyper oxygen-transmissible soft lenses, respectively, and 10.5% with the hyper oxygen-transmissible RGP lens (P < 0.001). Epithelial desquamation significantly decreased in all groups (P < 0.001). Tear LDH levels increased for all test lenses (P < 0.001). Conclusions: Increased PA binding induced by wear of a conventional soft lens material is significantly greater than that induced by the new hyper oxygen-transmissible soft silicone hydrogel lens during DW. However, both soft materials showed significant increases in PA binding as compared with baseline controls. By contrast, hyper oxygen-transmissible RGP lens DW did not increase PA binding significantly. Taken together, these findings suggest for the first time both an oxygen effect as well as a difference between soft and rigid lens types on PA binding in DW.
AB - Objective: To determine the effects of lens type and oxygen transmissibility on human corneal epithelium during daily lens wear (DW). Design: Prospective, randomized, double-masked, single-center, parallel treatment groups clinical trial. Participants: Two hundred forty-six patients fitted with: (1) high oxygen-transmissible soft lenses (n = 36), (2) hyper oxygen-transmissible soft lenses (n = 135), and (3) hyper oxygen-transmissible rigid gas-permeable (RGP) lenses (n = 75). Intervention: Irrigation chamber to collect exfoliated epithelial surface cells, confocal microscopy, and tear collection at baseline, 2 weeks, and 4 weeks of DW. Main Outcome Measures: (1) Pseudomonas aeruginosa (PA) binding to exfoliated corneal epithelial surface cells, (2) central epithelial thickness, (3) superficial epithelial cell area, (4) epithelial surface cell exfoliation, and (5) tear lactate dehydrogenase (LDH). Results: Four weeks of DW with the high oxygen-transmissible soft lens significantly increased PA binding from baseline 6.55 ± 3.01 to 8.75 ± 3.05 bacteria per epithelial cell (P < 0.01). By contrast, hyper oxygen-transmissible soft lens wear increased binding significantly less (6.13 ± 2.45 to 7.62 ± 3.06; P < 0.01), whereas hyper oxygen-transmissible RGP lens wear demonstrated no significant changes (5.91 ± 2.40 to 6.13 ± 2.17; P = 0.533). No significant change in central epithelial thickness was found after 4 weeks of DW in either soft lens; however, the epithelial thickness decreased by 9.8% (P < 0.001) with RGP lens wear. Epithelial cell surface area increased 3.3% and 4.1% with the high and hyper oxygen-transmissible soft lenses, respectively, and 10.5% with the hyper oxygen-transmissible RGP lens (P < 0.001). Epithelial desquamation significantly decreased in all groups (P < 0.001). Tear LDH levels increased for all test lenses (P < 0.001). Conclusions: Increased PA binding induced by wear of a conventional soft lens material is significantly greater than that induced by the new hyper oxygen-transmissible soft silicone hydrogel lens during DW. However, both soft materials showed significant increases in PA binding as compared with baseline controls. By contrast, hyper oxygen-transmissible RGP lens DW did not increase PA binding significantly. Taken together, these findings suggest for the first time both an oxygen effect as well as a difference between soft and rigid lens types on PA binding in DW.
UR - http://www.scopus.com/inward/record.url?scp=0034972703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034972703&partnerID=8YFLogxK
U2 - 10.1016/S0161-6420(01)00639-X
DO - 10.1016/S0161-6420(01)00639-X
M3 - Article
C2 - 11425688
AN - SCOPUS:0034972703
SN - 0161-6420
VL - 108
SP - 1279
EP - 1288
JO - Ophthalmology
JF - Ophthalmology
IS - 7
ER -