Efficacy of liposomes containing a lipophilic muramyl dipeptide derivative for activating the tumoricidal properties of alveolar macrophages in vivo

I. J. Fidler, S. Sone, W. E. Fogler, D. Smith, D. G. Braun, L. Tarcsay, R. H. Gisler, A. J. Schroit

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

We examined the activation to the tumoricidal state of normal F344 rat and C57BL/6 mouse alveolar macrophages (AM) by liposomes containing entrapped hydrophilic muramyl dipeptide (H-MDP) or a lipophilic muramyl dipeptide derivative (MTP-PE) inserted directly into the liposome membranes. The superiority of liposomes containing the lipophilic MTP-PE over liposomes containing H-MDP for in vitro and/or in vivo activation of AM was demonstrated in several experiments. First, the i.v. injection of liposomes containing a dose of MTP-PE equal to H-MDP led to higher levels of AM-mediated cytotoxicity as measured by in vitro assay. Second, AM harvested from mice given i.v. injections of liposomes containing MTP-PE maintained their tumoricidal activity for a longer period (5 days) than AM harvested from mice given i.v. injections of liposomes containing H-MDP (3 days). Similar results were obtained from experiments of AM activation in vitro. These data demonstrate that, for the in situ activation of AM, liposomes containing a lipophilic derivative of muramyl dipeptide are superior to liposomes containing hydrophilic muramyl dipeptide.

Original languageEnglish (US)
Pages (from-to)43-55
Number of pages13
JournalJournal of Biological Response Modifiers
Volume1
Issue number1
StatePublished - Jan 1 1982

Keywords

  • Liposomes
  • Macrophages
  • Muramyl dipeptide
  • Tumoricidal properties

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Cancer Research

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    Fidler, I. J., Sone, S., Fogler, W. E., Smith, D., Braun, D. G., Tarcsay, L., Gisler, R. H., & Schroit, A. J. (1982). Efficacy of liposomes containing a lipophilic muramyl dipeptide derivative for activating the tumoricidal properties of alveolar macrophages in vivo. Journal of Biological Response Modifiers, 1(1), 43-55.