Efficient and Sequence-Specific DNA-Templated Polymerization of Peptide Nucleic Acid Aldehydes

Daniel M. Rosenbaum, David R. Liu

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

On the basis of the distance-dependence of DNA-templated reductive amination reactions and of recent findings of D. Lynn and co-workers, we developed DNA-templated polymerizations of synthetic peptide nucleic acid (PNA) aldehydes. The coupling reactions proceed in a highly efficient and sequence-specific manner, even in the presence of mixtures of PNA aldehydes of different sequence. Synthetic peptide nucleic acid polymers containing as many as 40 PNA units (representing 10 consecutive coupling reactions) were formed efficiently. The ease of preparing PNAs containing tailor-made functional groups together with these findings raises the possibility of evolving synthetic sequence-defined polymers by iterated cycles of translation, selection, PCR amplification, and diversification previously available only to biological macromolecules.

Original languageEnglish (US)
Pages (from-to)13924-13925
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number46
DOIs
StatePublished - Nov 19 2003

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Peptide Nucleic Acids
Aldehydes
Polymerization
DNA
Polymers
Amination
Macromolecules
Functional groups
Amplification
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Efficient and Sequence-Specific DNA-Templated Polymerization of Peptide Nucleic Acid Aldehydes. / Rosenbaum, Daniel M.; Liu, David R.

In: Journal of the American Chemical Society, Vol. 125, No. 46, 19.11.2003, p. 13924-13925.

Research output: Contribution to journalArticle

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