Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor

Haiqi Chen, Sophia Liu, Samuel Padula, Daniel Lesman, Kettner Griswold, Allen Lin, Tongtong Zhao, Jamie L. Marshall, Fei Chen

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a MEK1 inhibitor-resistance screen, and identified functionally correlated mutations.

Original languageEnglish (US)
Pages (from-to)165-168
Number of pages4
JournalNature biotechnology
Volume38
Issue number2
DOIs
StatePublished - Feb 1 2020
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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