TY - JOUR
T1 - Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts
AU - Bogliotti, Yanina Soledad
AU - Wu, Jun
AU - Vilarino, Marcela
AU - Okamura, Daiji
AU - Soto, Delia Alba
AU - Zhong, Cuiqing
AU - Sakurai, Masahiro
AU - Sampaio, Rafael Vilar
AU - Suzuki, Keiichiro
AU - Izpisua Belmonte, Juan Carlos
AU - Ross, Pablo Juan
N1 - Funding Information:
We thank M. Schwarz and P. Schwarz for administrative help; the Salk Stem Cell Core for providing cell culture reagents; Trans Ova Genetics for providing OPU-derived embryos and performing SCNT from bESCs; and M. C. Ku, N. Hah, and T. Nguyen for RNA-seq experiments. Work in the P.J.R. laboratory was supported by NIH/Eunice Kennedy Shriver National Institute of Child Health and Human Development Grant R01- HD070044, United States Department of Agriculture (USDA)–National Institute of Food and Agriculture (NIFA)–Agriculture and Food Research Initiative (AFRI) Multistate Project W3171, and by a University of California, Davis Academic Senate New Research Grant. Y.S.B. was supported by National Science Foundation-Graduate Research Fellowship Program Award 1148897. R.V.S. was supported by São Paulo Research Foundation (FAPESP) Grants 2013/08135-2 and 2015/25111-5. Work in the J.C.I.B. laboratory was supported by the Universidad Católica San Antonio de Murcia, the G. Harold and Leila Y. Mathers Charitable Foundation, The Moxie Foundation, Fundación Dr. Pedro Guillén, and NIH Grant DP1- DK113616.
Funding Information:
ACKNOWLEDGMENTS. We thank M. Schwarz and P. Schwarz for administrative help; the Salk Stem Cell Core for providing cell culture reagents; Trans Ova Genetics for providing OPU-derived embryos and performing SCNT from bESCs; and M. C. Ku, N. Hah, and T. Nguyen for RNA-seq experiments. Work in the P.J.R. laboratory was supported by NIH/Eunice Kennedy Shriver National Institute of Child Health and Human Development Grant R01-HD070044, United States Department of Agriculture (USDA)–National Institute of Food and Agriculture (NIFA)–Agriculture and Food Research Initiative (AFRI) Multistate Project W3171, and by a University of California, Davis Academic Senate New Research Grant. Y.S.B. was supported by National Science Foundation-Graduate Research Fellowship Program Award 1148897. R.V.S. was supported by São Paulo Research Foundation (FAPESP) Grants 2013/08135-2 and 2015/25111-5. Work in the J.C.I.B. laboratory was supported by the Universidad Católica San Antonio de Murcia, the G. Harold and Leila Y. Mathers Charitable Foundation, The Moxie Foundation, Fundación Dr. Pedro Guillén, and NIH Grant DP1-DK113616.
PY - 2018/2/27
Y1 - 2018/2/27
N2 - Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3–4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.
AB - Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3–4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.
KW - Bovine
KW - Embryonic stem cell
KW - Inner cell mass
KW - Pluripotency
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U2 - 10.1073/pnas.1716161115
DO - 10.1073/pnas.1716161115
M3 - Article
C2 - 29440377
AN - SCOPUS:85042644612
SN - 0027-8424
VL - 115
SP - 2090
EP - 2095
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -