Efficient methods for attachment of thiol specific probes to the 3′-ends of synthetic oligodeoxyribonucleotides

Ronald Zuckermann, David Corey, Peter Schultz

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Methodology is described for the synthesis of DNA oligomers containing a free 3′-thiol group which can be selectively crosslinked with a wide variety of probes. This chemistry is compatible with both phosphotriester and phosphoramidite solid phase chemistry. Moreover, the sulphydryl group is introduced into the 3′-nucleoside solid support linkage prior to oligonucleotide synthesis. Consequently, no additional coupling steps are required after oligonucleotide synthesis, and isolation of the 3′-thiol oligonucleotide requires only one additional deprotection step. Cross-linking of the thiol-containing oligonucleotide to a fluorescent probe was carried out with high selectivity, in high yield, and under mild conditions.

Original languageEnglish (US)
Pages (from-to)5305-5321
Number of pages17
JournalNucleic Acids Research
Volume15
Issue number13
DOIs
StatePublished - Jul 10 1987

Fingerprint

Oligonucleotides
Oligodeoxyribonucleotides
Sulfhydryl Compounds
Probe
Synthesis
Chemistry
Selectivity
Linkage
Linking
Isolation
Fluorescent Dyes
Nucleosides
Oligomers
DNA
Methodology

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Efficient methods for attachment of thiol specific probes to the 3′-ends of synthetic oligodeoxyribonucleotides. / Zuckermann, Ronald; Corey, David; Schultz, Peter.

In: Nucleic Acids Research, Vol. 15, No. 13, 10.07.1987, p. 5305-5321.

Research output: Contribution to journalArticle

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