Elastin expression in very severe human COPD

G. Deslee, J. C. Woods, C. M. Moore, L. Liu, S. H. Conradi, M. Milne, D. S. Gierada, J. Pierce, A. Patterson, R. A. Lewit, J. T. Battaile, M. J. Holtzman, J. C. Hogg, R. A. Pierce

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Alveolar elastic fibres are key targets of proteases during the pathogenesis of chronic obstructive pulmonary disease (COPD). In the current study, we hypothesised that a response to injury leads to enhanced alveolar elastin gene expression in very severe COPD. Lung samples obtained from 43 patients, including 11 with very severe COPD (stage 4), 10 donors, 10 with moderate/severe COPD (stage 2-3) and 12 non-COPD subjects, were analysed for elastin mRNA expression by real-time RT-PCR and in situ hybridisation. Alveolar elastic fibres were visualised using Hart's staining of sections of frozen inflated lungs obtained from 11 COPD stage 4 patients and three donor lungs. Compared with donors, non-COPD and stage 2-3 COPD, elastin mRNA expression was significantly increased in very severe COPD lungs (12-fold change), and localised in situ hybridisation induced elastin expression to alveolar walls. Compared with donors, alveolar elastic fibres also comprised a greater volume fraction of total lung tissue in very severe COPD lungs (p,0.01), but elastic fibre content was not increased per lung volume, and desmosine content was not increased. The present study demonstrates enhanced alveolar elastin expression in very severe COPD. The efficiency of this potential repair mechanism and its regulation remain to be demonstrated. Copyright

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalEuropean Respiratory Journal
Volume34
Issue number2
DOIs
StatePublished - Aug 2008

Fingerprint

Elastin
Chronic Obstructive Pulmonary Disease
Elastic Tissue
Lung
Tissue Donors
Obstructive Lung Diseases
In Situ Hybridization
Desmosine
Messenger RNA
Frozen Sections
Real-Time Polymerase Chain Reaction
Peptide Hydrolases
Staining and Labeling
Gene Expression
Wounds and Injuries

Keywords

  • Chronic obstructive pulmonary disease
  • Elastin
  • Emphysema
  • Gene expression

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Deslee, G., Woods, J. C., Moore, C. M., Liu, L., Conradi, S. H., Milne, M., ... Pierce, R. A. (2008). Elastin expression in very severe human COPD. European Respiratory Journal, 34(2), 324-331. https://doi.org/10.1183/09031936.00123008

Elastin expression in very severe human COPD. / Deslee, G.; Woods, J. C.; Moore, C. M.; Liu, L.; Conradi, S. H.; Milne, M.; Gierada, D. S.; Pierce, J.; Patterson, A.; Lewit, R. A.; Battaile, J. T.; Holtzman, M. J.; Hogg, J. C.; Pierce, R. A.

In: European Respiratory Journal, Vol. 34, No. 2, 08.2008, p. 324-331.

Research output: Contribution to journalArticle

Deslee, G, Woods, JC, Moore, CM, Liu, L, Conradi, SH, Milne, M, Gierada, DS, Pierce, J, Patterson, A, Lewit, RA, Battaile, JT, Holtzman, MJ, Hogg, JC & Pierce, RA 2008, 'Elastin expression in very severe human COPD', European Respiratory Journal, vol. 34, no. 2, pp. 324-331. https://doi.org/10.1183/09031936.00123008
Deslee G, Woods JC, Moore CM, Liu L, Conradi SH, Milne M et al. Elastin expression in very severe human COPD. European Respiratory Journal. 2008 Aug;34(2):324-331. https://doi.org/10.1183/09031936.00123008
Deslee, G. ; Woods, J. C. ; Moore, C. M. ; Liu, L. ; Conradi, S. H. ; Milne, M. ; Gierada, D. S. ; Pierce, J. ; Patterson, A. ; Lewit, R. A. ; Battaile, J. T. ; Holtzman, M. J. ; Hogg, J. C. ; Pierce, R. A. / Elastin expression in very severe human COPD. In: European Respiratory Journal. 2008 ; Vol. 34, No. 2. pp. 324-331.
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