Elastin expression in very severe human COPD

G. Deslee, J. C. Woods, C. M. Moore, L. Liu, S. H. Conradi, M. Milne, D. S. Gierada, J. Pierce, A. Patterson, R. A. Lewit, J. T. Battaile, M. J. Holtzman, J. C. Hogg, R. A. Pierce

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Alveolar elastic fibres are key targets of proteases during the pathogenesis of chronic obstructive pulmonary disease (COPD). In the current study, we hypothesised that a response to injury leads to enhanced alveolar elastin gene expression in very severe COPD. Lung samples obtained from 43 patients, including 11 with very severe COPD (stage 4), 10 donors, 10 with moderate/severe COPD (stage 2-3) and 12 non-COPD subjects, were analysed for elastin mRNA expression by real-time RT-PCR and in situ hybridisation. Alveolar elastic fibres were visualised using Hart's staining of sections of frozen inflated lungs obtained from 11 COPD stage 4 patients and three donor lungs. Compared with donors, non-COPD and stage 2-3 COPD, elastin mRNA expression was significantly increased in very severe COPD lungs (12-fold change), and localised in situ hybridisation induced elastin expression to alveolar walls. Compared with donors, alveolar elastic fibres also comprised a greater volume fraction of total lung tissue in very severe COPD lungs (p,0.01), but elastic fibre content was not increased per lung volume, and desmosine content was not increased. The present study demonstrates enhanced alveolar elastin expression in very severe COPD. The efficiency of this potential repair mechanism and its regulation remain to be demonstrated. Copyright

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalEuropean Respiratory Journal
Volume34
Issue number2
DOIs
StatePublished - Aug 1 2008

Keywords

  • Chronic obstructive pulmonary disease
  • Elastin
  • Emphysema
  • Gene expression

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Elastin expression in very severe human COPD'. Together they form a unique fingerprint.

  • Cite this

    Deslee, G., Woods, J. C., Moore, C. M., Liu, L., Conradi, S. H., Milne, M., Gierada, D. S., Pierce, J., Patterson, A., Lewit, R. A., Battaile, J. T., Holtzman, M. J., Hogg, J. C., & Pierce, R. A. (2008). Elastin expression in very severe human COPD. European Respiratory Journal, 34(2), 324-331. https://doi.org/10.1183/09031936.00123008