Electric-field-stimulated protein mechanics

Doeke R. Hekstra, K. Ian White, Michael A. Socolich, Robert W. Henning, Vukica Šrajer, Rama Ranganathan

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The internal mechanics of proteins - the coordinated motions of amino acids and the pattern of forces constraining these motions - connects protein structure to function. Here we describe a new method combining the application of strong electric field pulses to protein crystals with time-resolved X-ray crystallography to observe conformational changes in spatial and temporal detail. Using a human PDZ domain (LNX2 PDZ2) as a model system, we show that protein crystals tolerate electric field pulses strong enough to drive concerted motions on the sub-microsecond timescale. The induced motions are subtle, involve diverse physical mechanisms, and occur throughout the protein structure. The global pattern of electric-field-induced motions is consistent with both local and allosteric conformational changes naturally induced by ligand binding, including at conserved functional sites in the PDZ domain family. This work lays the foundation for comprehensive experimental study of the mechanical basis of protein function.

Original languageEnglish (US)
Pages (from-to)400-405
Number of pages6
JournalNature
Volume540
Issue number7633
DOIs
StatePublished - Dec 15 2016

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Mechanics
PDZ Domains
Proteins
X Ray Crystallography
Ligands
Amino Acids

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Hekstra, D. R., White, K. I., Socolich, M. A., Henning, R. W., Šrajer, V., & Ranganathan, R. (2016). Electric-field-stimulated protein mechanics. Nature, 540(7633), 400-405. https://doi.org/10.1038/nature20571

Electric-field-stimulated protein mechanics. / Hekstra, Doeke R.; White, K. Ian; Socolich, Michael A.; Henning, Robert W.; Šrajer, Vukica; Ranganathan, Rama.

In: Nature, Vol. 540, No. 7633, 15.12.2016, p. 400-405.

Research output: Contribution to journalArticle

Hekstra, DR, White, KI, Socolich, MA, Henning, RW, Šrajer, V & Ranganathan, R 2016, 'Electric-field-stimulated protein mechanics', Nature, vol. 540, no. 7633, pp. 400-405. https://doi.org/10.1038/nature20571
Hekstra DR, White KI, Socolich MA, Henning RW, Šrajer V, Ranganathan R. Electric-field-stimulated protein mechanics. Nature. 2016 Dec 15;540(7633):400-405. https://doi.org/10.1038/nature20571
Hekstra, Doeke R. ; White, K. Ian ; Socolich, Michael A. ; Henning, Robert W. ; Šrajer, Vukica ; Ranganathan, Rama. / Electric-field-stimulated protein mechanics. In: Nature. 2016 ; Vol. 540, No. 7633. pp. 400-405.
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