Electroconvulsive Seizures Stimulate Glial Proliferation and Reduce Expression of Sprouty2 within the Prefrontal Cortex of Rats

Dost Öngür, Jessica Pohlman, Antonia L. Dow, Amelia J. Eisch, Francis Edwin, Stephan Heckers, Bruce M. Cohen, Tarun B. Patel, William A. Carlezon

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Reductions in cell number are found within the medial prefrontal cortex (PFC) in major depression and bipolar disorder, conditions for which electroconvulsive therapy (ECT) is a highly effective treatment. We investigated whether electroconvulsive seizure (ECS) in rats stimulates cellular proliferation in the PFC immediately and four weeks after the treatments. In parallel, we examined if ECS also alters the expression of Sprouty2 (SPRY2), an inhibitor of cell proliferation. Methods: Sprague-Dawley rats received 10 days of ECS treatments and bromodeoxyuridine (BrdU) injections. After a four week survival period, we estimated the density and number of BrdU-, proliferating cell nuclear antigen (PCNA)-, and SPRY2-immunoreactive cells in the medial (infralimbic) PFC (ILPFC). We also determined the percentage of BrdU-labeled cells that were immunoreactive for markers specific to oligodendrocytes, astrocytes, endothelial cells and neurons. Results: ECS dramatically enhanced the proliferation of new cells in the infralimbic PFC, and this effect persisted four weeks following the treatments. The percentage of new cells expressing oligodendrocyte precursor cell markers increased slightly following ECS. In contrast, ECS dramatically reduced the number of cells expressing SPRY2. Conclusions: ECS stimulates long-lasting increases in glial proliferation within the ILPFC. ECS also decreases SPRY2 expression in the same region, an effect that might contribute to increased glial proliferation.

Original languageEnglish (US)
Pages (from-to)505-512
Number of pages8
JournalBiological Psychiatry
Volume62
Issue number5
DOIs
StatePublished - Sep 1 2007

Fingerprint

Prefrontal Cortex
Neuroglia
Seizures
Bromodeoxyuridine
Oligodendroglia
Cell Proliferation
Cell Count
Electroconvulsive Therapy
Proliferating Cell Nuclear Antigen
Therapeutics
Bipolar Disorder
Astrocytes
Sprague Dawley Rats
Endothelial Cells
Depression
Neurons
Injections

Keywords

  • Bipolar disorder
  • depression
  • electroconvulsive seizure
  • glia
  • infralimbic prefrontal cortex
  • NG2
  • Sprouty

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Electroconvulsive Seizures Stimulate Glial Proliferation and Reduce Expression of Sprouty2 within the Prefrontal Cortex of Rats. / Öngür, Dost; Pohlman, Jessica; Dow, Antonia L.; Eisch, Amelia J.; Edwin, Francis; Heckers, Stephan; Cohen, Bruce M.; Patel, Tarun B.; Carlezon, William A.

In: Biological Psychiatry, Vol. 62, No. 5, 01.09.2007, p. 505-512.

Research output: Contribution to journalArticle

Öngür, Dost ; Pohlman, Jessica ; Dow, Antonia L. ; Eisch, Amelia J. ; Edwin, Francis ; Heckers, Stephan ; Cohen, Bruce M. ; Patel, Tarun B. ; Carlezon, William A. / Electroconvulsive Seizures Stimulate Glial Proliferation and Reduce Expression of Sprouty2 within the Prefrontal Cortex of Rats. In: Biological Psychiatry. 2007 ; Vol. 62, No. 5. pp. 505-512.
@article{fa6e3c30813041729b1ae5df97d6281b,
title = "Electroconvulsive Seizures Stimulate Glial Proliferation and Reduce Expression of Sprouty2 within the Prefrontal Cortex of Rats",
abstract = "Background: Reductions in cell number are found within the medial prefrontal cortex (PFC) in major depression and bipolar disorder, conditions for which electroconvulsive therapy (ECT) is a highly effective treatment. We investigated whether electroconvulsive seizure (ECS) in rats stimulates cellular proliferation in the PFC immediately and four weeks after the treatments. In parallel, we examined if ECS also alters the expression of Sprouty2 (SPRY2), an inhibitor of cell proliferation. Methods: Sprague-Dawley rats received 10 days of ECS treatments and bromodeoxyuridine (BrdU) injections. After a four week survival period, we estimated the density and number of BrdU-, proliferating cell nuclear antigen (PCNA)-, and SPRY2-immunoreactive cells in the medial (infralimbic) PFC (ILPFC). We also determined the percentage of BrdU-labeled cells that were immunoreactive for markers specific to oligodendrocytes, astrocytes, endothelial cells and neurons. Results: ECS dramatically enhanced the proliferation of new cells in the infralimbic PFC, and this effect persisted four weeks following the treatments. The percentage of new cells expressing oligodendrocyte precursor cell markers increased slightly following ECS. In contrast, ECS dramatically reduced the number of cells expressing SPRY2. Conclusions: ECS stimulates long-lasting increases in glial proliferation within the ILPFC. ECS also decreases SPRY2 expression in the same region, an effect that might contribute to increased glial proliferation.",
keywords = "Bipolar disorder, depression, electroconvulsive seizure, glia, infralimbic prefrontal cortex, NG2, Sprouty",
author = "Dost {\"O}ng{\"u}r and Jessica Pohlman and Dow, {Antonia L.} and Eisch, {Amelia J.} and Francis Edwin and Stephan Heckers and Cohen, {Bruce M.} and Patel, {Tarun B.} and Carlezon, {William A.}",
year = "2007",
month = "9",
day = "1",
doi = "10.1016/j.biopsych.2006.11.014",
language = "English (US)",
volume = "62",
pages = "505--512",
journal = "Biological Psychiatry",
issn = "0006-3223",
publisher = "Elsevier USA",
number = "5",

}

TY - JOUR

T1 - Electroconvulsive Seizures Stimulate Glial Proliferation and Reduce Expression of Sprouty2 within the Prefrontal Cortex of Rats

AU - Öngür, Dost

AU - Pohlman, Jessica

AU - Dow, Antonia L.

AU - Eisch, Amelia J.

AU - Edwin, Francis

AU - Heckers, Stephan

AU - Cohen, Bruce M.

AU - Patel, Tarun B.

AU - Carlezon, William A.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Background: Reductions in cell number are found within the medial prefrontal cortex (PFC) in major depression and bipolar disorder, conditions for which electroconvulsive therapy (ECT) is a highly effective treatment. We investigated whether electroconvulsive seizure (ECS) in rats stimulates cellular proliferation in the PFC immediately and four weeks after the treatments. In parallel, we examined if ECS also alters the expression of Sprouty2 (SPRY2), an inhibitor of cell proliferation. Methods: Sprague-Dawley rats received 10 days of ECS treatments and bromodeoxyuridine (BrdU) injections. After a four week survival period, we estimated the density and number of BrdU-, proliferating cell nuclear antigen (PCNA)-, and SPRY2-immunoreactive cells in the medial (infralimbic) PFC (ILPFC). We also determined the percentage of BrdU-labeled cells that were immunoreactive for markers specific to oligodendrocytes, astrocytes, endothelial cells and neurons. Results: ECS dramatically enhanced the proliferation of new cells in the infralimbic PFC, and this effect persisted four weeks following the treatments. The percentage of new cells expressing oligodendrocyte precursor cell markers increased slightly following ECS. In contrast, ECS dramatically reduced the number of cells expressing SPRY2. Conclusions: ECS stimulates long-lasting increases in glial proliferation within the ILPFC. ECS also decreases SPRY2 expression in the same region, an effect that might contribute to increased glial proliferation.

AB - Background: Reductions in cell number are found within the medial prefrontal cortex (PFC) in major depression and bipolar disorder, conditions for which electroconvulsive therapy (ECT) is a highly effective treatment. We investigated whether electroconvulsive seizure (ECS) in rats stimulates cellular proliferation in the PFC immediately and four weeks after the treatments. In parallel, we examined if ECS also alters the expression of Sprouty2 (SPRY2), an inhibitor of cell proliferation. Methods: Sprague-Dawley rats received 10 days of ECS treatments and bromodeoxyuridine (BrdU) injections. After a four week survival period, we estimated the density and number of BrdU-, proliferating cell nuclear antigen (PCNA)-, and SPRY2-immunoreactive cells in the medial (infralimbic) PFC (ILPFC). We also determined the percentage of BrdU-labeled cells that were immunoreactive for markers specific to oligodendrocytes, astrocytes, endothelial cells and neurons. Results: ECS dramatically enhanced the proliferation of new cells in the infralimbic PFC, and this effect persisted four weeks following the treatments. The percentage of new cells expressing oligodendrocyte precursor cell markers increased slightly following ECS. In contrast, ECS dramatically reduced the number of cells expressing SPRY2. Conclusions: ECS stimulates long-lasting increases in glial proliferation within the ILPFC. ECS also decreases SPRY2 expression in the same region, an effect that might contribute to increased glial proliferation.

KW - Bipolar disorder

KW - depression

KW - electroconvulsive seizure

KW - glia

KW - infralimbic prefrontal cortex

KW - NG2

KW - Sprouty

UR - http://www.scopus.com/inward/record.url?scp=34548009165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548009165&partnerID=8YFLogxK

U2 - 10.1016/j.biopsych.2006.11.014

DO - 10.1016/j.biopsych.2006.11.014

M3 - Article

C2 - 17336937

AN - SCOPUS:34548009165

VL - 62

SP - 505

EP - 512

JO - Biological Psychiatry

JF - Biological Psychiatry

SN - 0006-3223

IS - 5

ER -