Electron capture dissociation for robust production of sequence tags from eukaryotic proteins

Neil L. Kelleher, Steven Patrie, Jeffrey R. Johnson, Fanyu Meng, Andrew J. Forbes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The potential of electron capture dissociation (ECD) was translated into identification of heavily-modified proteins from eukaryotic proteins isolated from S. cerevisiae. Yeast cells were grown to stationary phase and collected by centrifugation. Cell lysis by sonication was followed by fractionation of the cell extract using a combination of continuous-elution PAGE and reversed-phase HPLC. It was found that ∼40-50% of fragment ions from standard threshold dissociation techniques do not occur in strings, for ions of ∼75 wild-type proteins.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages101-102
Number of pages2
Publication statusPublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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ASJC Scopus subject areas

  • Spectroscopy

Cite this

Kelleher, N. L., Patrie, S., Johnson, J. R., Meng, F., & Forbes, A. J. (2002). Electron capture dissociation for robust production of sequence tags from eukaryotic proteins. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 101-102)