Electron microscopy for the diagnosis of ocular viral infections

C. F. Boerner, F. K. Lee, C. L. Wickliffe, A. J. Nahmias, Harrison D Cavanagh, S. E. Straus

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tear samples obtained from patients with suspected viral conjunctivitis were examined by virus culture and by the pseudoreplica method of electron microscopy (EM). Adenoviruses were isolated in 13 of 34 cases (38%) clinically diagnosed as adenovirus conjunctivitis. Characteristic adenoviral particles were demonstrated by electron microscopy in seven of the culture-positive, and two of the culture-negative samples. In cases of herpes simplex virus ocular infection, tears were virus positive by culture in one-third of the patients; two-thirds of the culture positive specimens were also positive by EM. This study demonstrates the potential of electron microscopy as a rapid, specific, and relatively inexpensive method for the laboratory diagnosis of ocular viral infections.

Original languageEnglish (US)
Pages (from-to)1377-1381
Number of pages5
JournalOphthalmology
Volume88
Issue number12
StatePublished - 1981

Fingerprint

Eye Infections
Virus Diseases
Electron Microscopy
Tears
Adenoviridae
Viral Conjunctivitis
Viruses
Conjunctivitis
Clinical Laboratory Techniques
Simplexvirus

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Boerner, C. F., Lee, F. K., Wickliffe, C. L., Nahmias, A. J., Cavanagh, H. D., & Straus, S. E. (1981). Electron microscopy for the diagnosis of ocular viral infections. Ophthalmology, 88(12), 1377-1381.

Electron microscopy for the diagnosis of ocular viral infections. / Boerner, C. F.; Lee, F. K.; Wickliffe, C. L.; Nahmias, A. J.; Cavanagh, Harrison D; Straus, S. E.

In: Ophthalmology, Vol. 88, No. 12, 1981, p. 1377-1381.

Research output: Contribution to journalArticle

Boerner, CF, Lee, FK, Wickliffe, CL, Nahmias, AJ, Cavanagh, HD & Straus, SE 1981, 'Electron microscopy for the diagnosis of ocular viral infections', Ophthalmology, vol. 88, no. 12, pp. 1377-1381.
Boerner CF, Lee FK, Wickliffe CL, Nahmias AJ, Cavanagh HD, Straus SE. Electron microscopy for the diagnosis of ocular viral infections. Ophthalmology. 1981;88(12):1377-1381.
Boerner, C. F. ; Lee, F. K. ; Wickliffe, C. L. ; Nahmias, A. J. ; Cavanagh, Harrison D ; Straus, S. E. / Electron microscopy for the diagnosis of ocular viral infections. In: Ophthalmology. 1981 ; Vol. 88, No. 12. pp. 1377-1381.
@article{7ef02fd89f8047b286b662bb67672e2d,
title = "Electron microscopy for the diagnosis of ocular viral infections",
abstract = "Tear samples obtained from patients with suspected viral conjunctivitis were examined by virus culture and by the pseudoreplica method of electron microscopy (EM). Adenoviruses were isolated in 13 of 34 cases (38{\%}) clinically diagnosed as adenovirus conjunctivitis. Characteristic adenoviral particles were demonstrated by electron microscopy in seven of the culture-positive, and two of the culture-negative samples. In cases of herpes simplex virus ocular infection, tears were virus positive by culture in one-third of the patients; two-thirds of the culture positive specimens were also positive by EM. This study demonstrates the potential of electron microscopy as a rapid, specific, and relatively inexpensive method for the laboratory diagnosis of ocular viral infections.",
author = "Boerner, {C. F.} and Lee, {F. K.} and Wickliffe, {C. L.} and Nahmias, {A. J.} and Cavanagh, {Harrison D} and Straus, {S. E.}",
year = "1981",
language = "English (US)",
volume = "88",
pages = "1377--1381",
journal = "Ophthalmology",
issn = "0161-6420",
publisher = "Elsevier Inc.",
number = "12",

}

TY - JOUR

T1 - Electron microscopy for the diagnosis of ocular viral infections

AU - Boerner, C. F.

AU - Lee, F. K.

AU - Wickliffe, C. L.

AU - Nahmias, A. J.

AU - Cavanagh, Harrison D

AU - Straus, S. E.

PY - 1981

Y1 - 1981

N2 - Tear samples obtained from patients with suspected viral conjunctivitis were examined by virus culture and by the pseudoreplica method of electron microscopy (EM). Adenoviruses were isolated in 13 of 34 cases (38%) clinically diagnosed as adenovirus conjunctivitis. Characteristic adenoviral particles were demonstrated by electron microscopy in seven of the culture-positive, and two of the culture-negative samples. In cases of herpes simplex virus ocular infection, tears were virus positive by culture in one-third of the patients; two-thirds of the culture positive specimens were also positive by EM. This study demonstrates the potential of electron microscopy as a rapid, specific, and relatively inexpensive method for the laboratory diagnosis of ocular viral infections.

AB - Tear samples obtained from patients with suspected viral conjunctivitis were examined by virus culture and by the pseudoreplica method of electron microscopy (EM). Adenoviruses were isolated in 13 of 34 cases (38%) clinically diagnosed as adenovirus conjunctivitis. Characteristic adenoviral particles were demonstrated by electron microscopy in seven of the culture-positive, and two of the culture-negative samples. In cases of herpes simplex virus ocular infection, tears were virus positive by culture in one-third of the patients; two-thirds of the culture positive specimens were also positive by EM. This study demonstrates the potential of electron microscopy as a rapid, specific, and relatively inexpensive method for the laboratory diagnosis of ocular viral infections.

UR - http://www.scopus.com/inward/record.url?scp=0019840134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019840134&partnerID=8YFLogxK

M3 - Article

C2 - 6275326

AN - SCOPUS:0019840134

VL - 88

SP - 1377

EP - 1381

JO - Ophthalmology

JF - Ophthalmology

SN - 0161-6420

IS - 12

ER -