Electrophoretic analyses of nucleosomes and other protein-DNA complexes

S. Y. Huang, W. T. Garrard

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Electrophoresis has proven to be a powerful resolving technique for both analytical and preparative biochemical experiments. Electrophoretic resolution of ribosome and polysome components was successfully achieved. This chapter discusses the following topics: (1) one-dimensional electrophoretic resolution of nucleosomes, (2) enzymatic preparation of chromatin and chromatin fractionation, (3) two-dimensional electrophoretic resolution of DNA and detection of specific sequences, (4) two-dimensional electrophoretic resolution of proteins using Triton-acid-urea gels, and (5) further applications for electrophoretic analyses. This chapter also have described techniques to (1) determine the overall electrophoretie patterns of various nucleoprotein species, (2) analyze the bulk DNA lengths of such species by performing a second dimension of electrophoresis in the presence of SDS, (3) determine the position of specific sequences in such two-dimensional displays after DNA transfer and nucleic acid hybridization, and (4) analyze the bulk protein compositions of such species by performing a second dimension of electrophoresis in Triton-acidurea gels.

Original languageEnglish (US)
Pages (from-to)116-142
Number of pages27
JournalMethods in Enzymology
Volume170
Issue numberC
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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