TY - JOUR
T1 - Electrophysiological characterization of human distal colon epithelium isolated using a novel technique
AU - Marrero, Jorge A.
AU - Ostrovskiy, Denis A.
AU - Matkowskyj, Kristina A.
AU - Koutsouris, Sia
AU - Hecht, Gail
AU - Benya, Richard V.
N1 - Funding Information:
This work was supported in part by National Institutes of Health grant DK-15168, a Veterans Administration Merit Review, and an ADHF/American Gastroenterological Association Industry Research Scholar Award to R.V.B.; and by National Institutes of Health grant DK-05694, and a Veterans Administration Merit Review to G.H.
PY - 1998
Y1 - 1998
N2 - Electrophysiological studies of human colonic epithelia traditionally have been hampered by the lack of tissue availability and by poor tissue quality. Human colonic epithelium is usually obtained surgically from individuals with underlying disease, while surgery itself can injure or alter the resected tissue. As a result, a wide range in electrophysiological parameters is reported in previous studies of human colonic epithelium. Such factors may also account for differences in measurements between humans and the few other species studied. We therefore devised a novel and rapid endoscopic technique, endoscopic mucosal resection (EMR), that allows for the removal and study of intestinal mucosal epithelium from normal volunteers. Using EMR we rapidly (7.2 ± 2.4 min) isolated surgical-sized epithelial sheets from the distal colon (1.4 ± 0.4 by 1.3 ± 0.4 cm) that were readily mounted in a 0.64-cm2 Ussing chamber. We observed stable resistance (289 ± 30 Ω · cm2), potential difference (1.6 ± 0.6 mV), and I(SC) (24 ± 9 μA/cm2) for at least 90 min, after which all experiments were terminated. Exposure to carbachol increased I(SC) 2.2 ± 0.5-fold, while forskolin increased I(SC) 4.4 ± 0.5-fold. These data show that the electrophysiological characteristics of the human distal colon removed by EMR more closely approximate values reported for other mammals than when removed using other techniques. Thus EMR represents a significant advance over traditional techniques for isolating human tissues and will increase the availability of this tissue for future studies.
AB - Electrophysiological studies of human colonic epithelia traditionally have been hampered by the lack of tissue availability and by poor tissue quality. Human colonic epithelium is usually obtained surgically from individuals with underlying disease, while surgery itself can injure or alter the resected tissue. As a result, a wide range in electrophysiological parameters is reported in previous studies of human colonic epithelium. Such factors may also account for differences in measurements between humans and the few other species studied. We therefore devised a novel and rapid endoscopic technique, endoscopic mucosal resection (EMR), that allows for the removal and study of intestinal mucosal epithelium from normal volunteers. Using EMR we rapidly (7.2 ± 2.4 min) isolated surgical-sized epithelial sheets from the distal colon (1.4 ± 0.4 by 1.3 ± 0.4 cm) that were readily mounted in a 0.64-cm2 Ussing chamber. We observed stable resistance (289 ± 30 Ω · cm2), potential difference (1.6 ± 0.6 mV), and I(SC) (24 ± 9 μA/cm2) for at least 90 min, after which all experiments were terminated. Exposure to carbachol increased I(SC) 2.2 ± 0.5-fold, while forskolin increased I(SC) 4.4 ± 0.5-fold. These data show that the electrophysiological characteristics of the human distal colon removed by EMR more closely approximate values reported for other mammals than when removed using other techniques. Thus EMR represents a significant advance over traditional techniques for isolating human tissues and will increase the availability of this tissue for future studies.
KW - Colonic epithelium
KW - Endoscopic mucosal resection
KW - Ion flux
KW - Short circuit current
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U2 - 10.1023/A:1026678030939
DO - 10.1023/A:1026678030939
M3 - Article
C2 - 9824131
AN - SCOPUS:0031796390
SN - 0163-2116
VL - 43
SP - 2439
EP - 2445
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 11
ER -