Electrophysiological characterization of neuromuscular synaptic dysfunction in mice

Yoshie Sugiura, Fujun Chen, Yun Liu, Weichun Lin

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Emerging evidence suggests that synaptic dysfunction occurs prior to neuronal loss in neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS). Therefore, monitoring synaptic activity during early stages of neurodegeneration may provide valuable information for the development of diagnostic and/or therapeutic strategies. Here, we describe an electrophysiological method routinely applied in our laboratory for investigating synaptic activity of the neuromuscular junction (NMJ), the synaptic connection between motoneurons and skeletal muscles. Using conventional intracellular sharp electrodes, both spontaneous synaptic activity (miniature end-plate potentials) and evoked synaptic activity (end-plate potentials) can be readily recorded in acutely isolated nerve-muscle preparations. This method can also be adapted to various simulation protocols for studying short-term plasticity of neuromuscular synapses.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages391-400
Number of pages10
Volume793
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume793
ISSN (Print)10643745

Fingerprint

Neuromuscular Junction
Miniature Postsynaptic Potentials
Excitatory Postsynaptic Potentials
Amyotrophic Lateral Sclerosis
Motor Neurons
Neurodegenerative Diseases
Synapses
Electrodes
Skeletal Muscle
Therapeutics

Keywords

  • Amyotrophic lateral sclerosis
  • End-plate potential
  • Intracellular recording
  • Miniature end-plate potential
  • Neurodegeneration
  • Neuromuscular junction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sugiura, Y., Chen, F., Liu, Y., & Lin, W. (2011). Electrophysiological characterization of neuromuscular synaptic dysfunction in mice. In Methods in Molecular Biology (Vol. 793, pp. 391-400). (Methods in Molecular Biology; Vol. 793). https://doi.org/10.1007/978-1-61779-328-8_26

Electrophysiological characterization of neuromuscular synaptic dysfunction in mice. / Sugiura, Yoshie; Chen, Fujun; Liu, Yun; Lin, Weichun.

Methods in Molecular Biology. Vol. 793 2011. p. 391-400 (Methods in Molecular Biology; Vol. 793).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sugiura, Y, Chen, F, Liu, Y & Lin, W 2011, Electrophysiological characterization of neuromuscular synaptic dysfunction in mice. in Methods in Molecular Biology. vol. 793, Methods in Molecular Biology, vol. 793, pp. 391-400. https://doi.org/10.1007/978-1-61779-328-8_26
Sugiura Y, Chen F, Liu Y, Lin W. Electrophysiological characterization of neuromuscular synaptic dysfunction in mice. In Methods in Molecular Biology. Vol. 793. 2011. p. 391-400. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-328-8_26
Sugiura, Yoshie ; Chen, Fujun ; Liu, Yun ; Lin, Weichun. / Electrophysiological characterization of neuromuscular synaptic dysfunction in mice. Methods in Molecular Biology. Vol. 793 2011. pp. 391-400 (Methods in Molecular Biology).
@inbook{a8bf3a415f024f74b2fbc4a27a8f3b20,
title = "Electrophysiological characterization of neuromuscular synaptic dysfunction in mice",
abstract = "Emerging evidence suggests that synaptic dysfunction occurs prior to neuronal loss in neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS). Therefore, monitoring synaptic activity during early stages of neurodegeneration may provide valuable information for the development of diagnostic and/or therapeutic strategies. Here, we describe an electrophysiological method routinely applied in our laboratory for investigating synaptic activity of the neuromuscular junction (NMJ), the synaptic connection between motoneurons and skeletal muscles. Using conventional intracellular sharp electrodes, both spontaneous synaptic activity (miniature end-plate potentials) and evoked synaptic activity (end-plate potentials) can be readily recorded in acutely isolated nerve-muscle preparations. This method can also be adapted to various simulation protocols for studying short-term plasticity of neuromuscular synapses.",
keywords = "Amyotrophic lateral sclerosis, End-plate potential, Intracellular recording, Miniature end-plate potential, Neurodegeneration, Neuromuscular junction",
author = "Yoshie Sugiura and Fujun Chen and Yun Liu and Weichun Lin",
year = "2011",
doi = "10.1007/978-1-61779-328-8_26",
language = "English (US)",
isbn = "9781617793271",
volume = "793",
series = "Methods in Molecular Biology",
pages = "391--400",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Electrophysiological characterization of neuromuscular synaptic dysfunction in mice

AU - Sugiura, Yoshie

AU - Chen, Fujun

AU - Liu, Yun

AU - Lin, Weichun

PY - 2011

Y1 - 2011

N2 - Emerging evidence suggests that synaptic dysfunction occurs prior to neuronal loss in neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS). Therefore, monitoring synaptic activity during early stages of neurodegeneration may provide valuable information for the development of diagnostic and/or therapeutic strategies. Here, we describe an electrophysiological method routinely applied in our laboratory for investigating synaptic activity of the neuromuscular junction (NMJ), the synaptic connection between motoneurons and skeletal muscles. Using conventional intracellular sharp electrodes, both spontaneous synaptic activity (miniature end-plate potentials) and evoked synaptic activity (end-plate potentials) can be readily recorded in acutely isolated nerve-muscle preparations. This method can also be adapted to various simulation protocols for studying short-term plasticity of neuromuscular synapses.

AB - Emerging evidence suggests that synaptic dysfunction occurs prior to neuronal loss in neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS). Therefore, monitoring synaptic activity during early stages of neurodegeneration may provide valuable information for the development of diagnostic and/or therapeutic strategies. Here, we describe an electrophysiological method routinely applied in our laboratory for investigating synaptic activity of the neuromuscular junction (NMJ), the synaptic connection between motoneurons and skeletal muscles. Using conventional intracellular sharp electrodes, both spontaneous synaptic activity (miniature end-plate potentials) and evoked synaptic activity (end-plate potentials) can be readily recorded in acutely isolated nerve-muscle preparations. This method can also be adapted to various simulation protocols for studying short-term plasticity of neuromuscular synapses.

KW - Amyotrophic lateral sclerosis

KW - End-plate potential

KW - Intracellular recording

KW - Miniature end-plate potential

KW - Neurodegeneration

KW - Neuromuscular junction

UR - http://www.scopus.com/inward/record.url?scp=80054773601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054773601&partnerID=8YFLogxK

U2 - 10.1007/978-1-61779-328-8_26

DO - 10.1007/978-1-61779-328-8_26

M3 - Chapter

C2 - 21913115

AN - SCOPUS:80054773601

SN - 9781617793271

VL - 793

T3 - Methods in Molecular Biology

SP - 391

EP - 400

BT - Methods in Molecular Biology

ER -