Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.

Translated title of the contribution: Electrostatic method of determination of phospholipase A activity

V. V. Chernyǐ, V. M. Mirskiǐ, V. S. Sokolov, V. S. Markin

Research output: Contribution to journalArticle

Abstract

An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.

Original languageUndefined
Pages (from-to)445-450
Number of pages6
JournalBiokhimiya
Volume55
Issue number3
StatePublished - Mar 1990

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Phospholipases
Phospholipases A
Static Electricity
Electrostatics
Assays
Thermodynamic properties
Lipid bilayers
Enzyme activity
Lipid Bilayers
Hydrolysis
Impurities
Monitoring
Substrates
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)

Cite this

Chernyǐ, V. V., Mirskiǐ, V. M., Sokolov, V. S., & Markin, V. S. (1990). Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A. Biokhimiya, 55(3), 445-450.

Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A. / Chernyǐ, V. V.; Mirskiǐ, V. M.; Sokolov, V. S.; Markin, V. S.

In: Biokhimiya, Vol. 55, No. 3, 03.1990, p. 445-450.

Research output: Contribution to journalArticle

Chernyǐ, VV, Mirskiǐ, VM, Sokolov, VS & Markin, VS 1990, 'Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.', Biokhimiya, vol. 55, no. 3, pp. 445-450.
Chernyǐ VV, Mirskiǐ VM, Sokolov VS, Markin VS. Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A. Biokhimiya. 1990 Mar;55(3):445-450.
Chernyǐ, V. V. ; Mirskiǐ, V. M. ; Sokolov, V. S. ; Markin, V. S. / Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A. In: Biokhimiya. 1990 ; Vol. 55, No. 3. pp. 445-450.
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