Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A.

Translated title of the contribution: Electrostatic method of determination of phospholipase A activity

V. V. Chernyǐ, V. M. Mirskiǐ, V. S. Sokolov, V. S. Markin

Research output: Contribution to journalArticle

Abstract

An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.

Translated title of the contributionElectrostatic method of determination of phospholipase A activity
Original languageRussian
Pages (from-to)445-450
Number of pages6
JournalBiokhimiya
Volume55
Issue number3
StatePublished - Mar 1 1990

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint Dive into the research topics of 'Electrostatic method of determination of phospholipase A activity'. Together they form a unique fingerprint.

  • Cite this

    Chernyǐ, V. V., Mirskiǐ, V. M., Sokolov, V. S., & Markin, V. S. (1990). Elektrostaticheskiǐ metod opredeleniia aktivnosti fosfolipazy A. Biokhimiya, 55(3), 445-450.