Elestrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials

Vladimir M. Mirsky; Vladimir V. Cherny; Valerij S. Sokolov; Vladislav S. Markin

doi:10.1016/0165-022X(90)90002-T

Elestrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials

Vladimir M. Mirsky, Vladimir V. Cherny, Valerij S. Sokolov, Vladislav S. Markin

Neurology & Neurotherapeutics

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential of the planar bilayer during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. On eanalysis takes about 5 min.

Original language	English (US)
Pages (from-to)	277-284
Number of pages	8
Journal	Journal of Biochemical and Biophysical Methods
Volume	21
Issue number	4
DOIs	https://doi.org/10.1016/0165-022X(90)90002-T
State	Published - 1990

Keywords

Bilayer lipid membrane
Boundary
Enzyme assay
Phospholipase A
Surface potential

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/0165-022X(90)90002-T

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title = "Elestrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials",

abstract = "To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential of the planar bilayer during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. On eanalysis takes about 5 min.",

keywords = "Bilayer lipid membrane, Boundary, Enzyme assay, Phospholipase A, Surface potential",

author = "Mirsky, {Vladimir M.} and Cherny, {Vladimir V.} and Sokolov, {Valerij S.} and Markin, {Vladislav S.}",

year = "1990",

doi = "10.1016/0165-022X(90)90002-T",

language = "English (US)",

volume = "21",

pages = "277--284",

journal = "Journal of Biochemical and Biophysical Methods",

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TY - JOUR

T1 - Elestrostatic assay of phospholipase A activity

T2 - an application of the second harmonic method of monitoring membrane boundary potentials

AU - Mirsky, Vladimir M.

AU - Cherny, Vladimir V.

AU - Sokolov, Valerij S.

AU - Markin, Vladislav S.

PY - 1990

Y1 - 1990

N2 - To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential of the planar bilayer during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. On eanalysis takes about 5 min.

AB - To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential of the planar bilayer during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. On eanalysis takes about 5 min.

KW - Bilayer lipid membrane

KW - Boundary

KW - Enzyme assay

KW - Phospholipase A

KW - Surface potential

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U2 - 10.1016/0165-022X(90)90002-T

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AN - SCOPUS:0025009231

SN - 0165-022X

VL - 21

SP - 277

EP - 284

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 4

ER -

Elestrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials

Abstract

Keywords

ASJC Scopus subject areas

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