Elestrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials

Vladimir M. Mirsky, Vladimir V. Cherny, Valerij S. Sokolov, Vladislav S. Markin

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential of the planar bilayer during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. On eanalysis takes about 5 min.

Original languageEnglish (US)
Pages (from-to)277-284
Number of pages8
JournalJournal of Biochemical and Biophysical Methods
Volume21
Issue number4
DOIs
Publication statusPublished - 1990

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Keywords

  • Bilayer lipid membrane
  • Boundary
  • Enzyme assay
  • Phospholipase A
  • Surface potential

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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