Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers

Dalibor Kurepa, Arun K. Pramanik, Venkatakrishna Kakkilaya, Gloria Caldito, Lynn J. Groome, Joseph A. Bocchini, Sushil K. Jain

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Infants of diabetic mothers (IDMs) are at increased risk for metabolic complications. Type 1 and some type 2 diabetic patients have elevated levels of the ketone bodies acetoacetate (AA) and β-hydroxybutyrate (BHB). Objective: The aim of this study was to examine how hyperketonemia in diabetic mothers affects markers of inflammation and oxidative stress in their offspring. Methods: Blood was obtained from 23 diabetic mothers and 13 healthy mothers and their infants' umbilical cords at delivery. Interleukin-8, monocyte chemotactic protein-1 (MCP-1) and protein carbonyl (protein oxidation) levels were determined by ELISA. U937 human monocyte cell culture was used to examine the effect of AA and BHB on secretion of MCP-1. Results: There was a significant increase in the levels of AA in cord blood of IDMs compared with cord blood of infants of healthy mothers. A significant increase in the levels of protein oxidation (p < 0.05) and MCP-1 levels (p < 0.05) was observed in the cord blood of IDMs. The level of MCP-1 correlated significantly (r = 0.51, p = 0.01) with the concentration of AA in the IDMs. In further experiments with cultured monocytes treated with exogenous AA (0-4 mM), a significant increase in MCP-1 secretion was observed in AA- but not BHB-treated monocytes. Conclusion: Blood levels of AA and MCP-1 are elevated in IDMs, which may contribute to the development of the metabolic complications seen in IDMs.

Original languageEnglish (US)
Pages (from-to)163-168
Number of pages6
JournalNeonatology
Volume102
Issue number3
DOIs
StatePublished - Sep 2012

Fingerprint

Chemokine CCL2
Fetal Blood
Mothers
Monocytes
acetoacetic acid
Hydroxybutyrates
Ketone Bodies
Proteins
Umbilical Cord
Interleukin-8
Oxidative Stress
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Inflammation

Keywords

  • Acetoacetate
  • Hyperketonemia
  • Infant of diabetic mother
  • Monocyte chemotactic protein-1
  • Oxidative stress

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

Kurepa, D., Pramanik, A. K., Kakkilaya, V., Caldito, G., Groome, L. J., Bocchini, J. A., & Jain, S. K. (2012). Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers. Neonatology, 102(3), 163-168. https://doi.org/10.1159/000339286

Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers. / Kurepa, Dalibor; Pramanik, Arun K.; Kakkilaya, Venkatakrishna; Caldito, Gloria; Groome, Lynn J.; Bocchini, Joseph A.; Jain, Sushil K.

In: Neonatology, Vol. 102, No. 3, 09.2012, p. 163-168.

Research output: Contribution to journalArticle

Kurepa, D, Pramanik, AK, Kakkilaya, V, Caldito, G, Groome, LJ, Bocchini, JA & Jain, SK 2012, 'Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers', Neonatology, vol. 102, no. 3, pp. 163-168. https://doi.org/10.1159/000339286
Kurepa, Dalibor ; Pramanik, Arun K. ; Kakkilaya, Venkatakrishna ; Caldito, Gloria ; Groome, Lynn J. ; Bocchini, Joseph A. ; Jain, Sushil K. / Elevated acetoacetate and monocyte chemotactic protein-1 levels in cord blood of infants of diabetic mothers. In: Neonatology. 2012 ; Vol. 102, No. 3. pp. 163-168.
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