Elevated second-trimester maternal serum hCG: A marker of inadequate angiogenesis

Catherine Y. Spong, Alessandro Ghidini, Gary A. Dildy, Carol A. Loucks, Michael W. Varner, John C. Pezzullo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To measure angiogenin, a potent inducer of neovascularization and interleukin-6, as an indicator of acute inflammation, in second-trimester amniotic fluid of patients with elevated maternal serum hCG. Methods: In this case-control study, 20 patients with elevated maternal serum hCG (at least 2.0 multiples of median) at triple screen were matched 2:1 with controls on the basis of year of amniocentesis, parity, and race. Inclusion criteria were 1) singleton gestation, 2) no evidence of anomalies, and 3) genetic amniocentesis. Amniotic fluid was immunoassayed for angiogenin and interleukin-6. The immunoassay sensitivity for angiogenin was 0.026 ng/mL, interassay coefficient of variation 4.6%, and intra-assay coefficient of variation 2.9%. For interleukin-6, the immunoassay sensitivity was 2.37 pg/mL, interassay coefficient of variation 2.7%, and intra-assay coefficient of variation 1.9%. Angiogenin and interleukin-6 values were normalized by using natural log transformation for statistical analysis. Statistical analysis included analysis of variance and stepwise regression, with P < .05 significant. Results: After correcting (by multivariate regression) for gestational age at sampling and nulliparity, amniotic fluid angiogenin levels were significantly lower in the study subjects than in controls (26% ± 11% lower, P = .004), whereas the interleukin-6 levels did not change significantly (34% ± 40% lower, P = .3). Conclusion: Amniotic fluid angiogenin levels are significantly lower in patients with elevated maternal serum hCG at triple screen, suggesting inadequate angiogenesis, but interleukin-6 values do not differ significantly.

Original languageEnglish (US)
Pages (from-to)605-608
Number of pages4
JournalObstetrics and gynecology
Volume91
Issue number4
DOIs
StatePublished - Apr 1 1998
Externally publishedYes

Fingerprint

Second Pregnancy Trimester
Interleukin-6
Mothers
Amniotic Fluid
Serum
Amniocentesis
Parity
Immunoassay
Gestational Age
angiogenin
Case-Control Studies
Analysis of Variance
Inflammation
Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Elevated second-trimester maternal serum hCG : A marker of inadequate angiogenesis. / Spong, Catherine Y.; Ghidini, Alessandro; Dildy, Gary A.; Loucks, Carol A.; Varner, Michael W.; Pezzullo, John C.

In: Obstetrics and gynecology, Vol. 91, No. 4, 01.04.1998, p. 605-608.

Research output: Contribution to journalArticle

Spong, Catherine Y. ; Ghidini, Alessandro ; Dildy, Gary A. ; Loucks, Carol A. ; Varner, Michael W. ; Pezzullo, John C. / Elevated second-trimester maternal serum hCG : A marker of inadequate angiogenesis. In: Obstetrics and gynecology. 1998 ; Vol. 91, No. 4. pp. 605-608.
@article{b8c948d895a743d78a94d82a8b05e746,
title = "Elevated second-trimester maternal serum hCG: A marker of inadequate angiogenesis",
abstract = "Objective: To measure angiogenin, a potent inducer of neovascularization and interleukin-6, as an indicator of acute inflammation, in second-trimester amniotic fluid of patients with elevated maternal serum hCG. Methods: In this case-control study, 20 patients with elevated maternal serum hCG (at least 2.0 multiples of median) at triple screen were matched 2:1 with controls on the basis of year of amniocentesis, parity, and race. Inclusion criteria were 1) singleton gestation, 2) no evidence of anomalies, and 3) genetic amniocentesis. Amniotic fluid was immunoassayed for angiogenin and interleukin-6. The immunoassay sensitivity for angiogenin was 0.026 ng/mL, interassay coefficient of variation 4.6{\%}, and intra-assay coefficient of variation 2.9{\%}. For interleukin-6, the immunoassay sensitivity was 2.37 pg/mL, interassay coefficient of variation 2.7{\%}, and intra-assay coefficient of variation 1.9{\%}. Angiogenin and interleukin-6 values were normalized by using natural log transformation for statistical analysis. Statistical analysis included analysis of variance and stepwise regression, with P < .05 significant. Results: After correcting (by multivariate regression) for gestational age at sampling and nulliparity, amniotic fluid angiogenin levels were significantly lower in the study subjects than in controls (26{\%} ± 11{\%} lower, P = .004), whereas the interleukin-6 levels did not change significantly (34{\%} ± 40{\%} lower, P = .3). Conclusion: Amniotic fluid angiogenin levels are significantly lower in patients with elevated maternal serum hCG at triple screen, suggesting inadequate angiogenesis, but interleukin-6 values do not differ significantly.",
author = "Spong, {Catherine Y.} and Alessandro Ghidini and Dildy, {Gary A.} and Loucks, {Carol A.} and Varner, {Michael W.} and Pezzullo, {John C.}",
year = "1998",
month = "4",
day = "1",
doi = "10.1016/S0029-7844(98)00010-6",
language = "English (US)",
volume = "91",
pages = "605--608",
journal = "Obstetrics and Gynecology",
issn = "0029-7844",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Elevated second-trimester maternal serum hCG

T2 - A marker of inadequate angiogenesis

AU - Spong, Catherine Y.

AU - Ghidini, Alessandro

AU - Dildy, Gary A.

AU - Loucks, Carol A.

AU - Varner, Michael W.

AU - Pezzullo, John C.

PY - 1998/4/1

Y1 - 1998/4/1

N2 - Objective: To measure angiogenin, a potent inducer of neovascularization and interleukin-6, as an indicator of acute inflammation, in second-trimester amniotic fluid of patients with elevated maternal serum hCG. Methods: In this case-control study, 20 patients with elevated maternal serum hCG (at least 2.0 multiples of median) at triple screen were matched 2:1 with controls on the basis of year of amniocentesis, parity, and race. Inclusion criteria were 1) singleton gestation, 2) no evidence of anomalies, and 3) genetic amniocentesis. Amniotic fluid was immunoassayed for angiogenin and interleukin-6. The immunoassay sensitivity for angiogenin was 0.026 ng/mL, interassay coefficient of variation 4.6%, and intra-assay coefficient of variation 2.9%. For interleukin-6, the immunoassay sensitivity was 2.37 pg/mL, interassay coefficient of variation 2.7%, and intra-assay coefficient of variation 1.9%. Angiogenin and interleukin-6 values were normalized by using natural log transformation for statistical analysis. Statistical analysis included analysis of variance and stepwise regression, with P < .05 significant. Results: After correcting (by multivariate regression) for gestational age at sampling and nulliparity, amniotic fluid angiogenin levels were significantly lower in the study subjects than in controls (26% ± 11% lower, P = .004), whereas the interleukin-6 levels did not change significantly (34% ± 40% lower, P = .3). Conclusion: Amniotic fluid angiogenin levels are significantly lower in patients with elevated maternal serum hCG at triple screen, suggesting inadequate angiogenesis, but interleukin-6 values do not differ significantly.

AB - Objective: To measure angiogenin, a potent inducer of neovascularization and interleukin-6, as an indicator of acute inflammation, in second-trimester amniotic fluid of patients with elevated maternal serum hCG. Methods: In this case-control study, 20 patients with elevated maternal serum hCG (at least 2.0 multiples of median) at triple screen were matched 2:1 with controls on the basis of year of amniocentesis, parity, and race. Inclusion criteria were 1) singleton gestation, 2) no evidence of anomalies, and 3) genetic amniocentesis. Amniotic fluid was immunoassayed for angiogenin and interleukin-6. The immunoassay sensitivity for angiogenin was 0.026 ng/mL, interassay coefficient of variation 4.6%, and intra-assay coefficient of variation 2.9%. For interleukin-6, the immunoassay sensitivity was 2.37 pg/mL, interassay coefficient of variation 2.7%, and intra-assay coefficient of variation 1.9%. Angiogenin and interleukin-6 values were normalized by using natural log transformation for statistical analysis. Statistical analysis included analysis of variance and stepwise regression, with P < .05 significant. Results: After correcting (by multivariate regression) for gestational age at sampling and nulliparity, amniotic fluid angiogenin levels were significantly lower in the study subjects than in controls (26% ± 11% lower, P = .004), whereas the interleukin-6 levels did not change significantly (34% ± 40% lower, P = .3). Conclusion: Amniotic fluid angiogenin levels are significantly lower in patients with elevated maternal serum hCG at triple screen, suggesting inadequate angiogenesis, but interleukin-6 values do not differ significantly.

UR - http://www.scopus.com/inward/record.url?scp=0032054891&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032054891&partnerID=8YFLogxK

U2 - 10.1016/S0029-7844(98)00010-6

DO - 10.1016/S0029-7844(98)00010-6

M3 - Article

C2 - 9540950

AN - SCOPUS:0032054891

VL - 91

SP - 605

EP - 608

JO - Obstetrics and Gynecology

JF - Obstetrics and Gynecology

SN - 0029-7844

IS - 4

ER -