Elimination of T cells from human peripheral blood and bone marrow using a cocktail of three anti-T cell immunotoxins

F. E. Katz, G. Janossy, A. Cumber, W. Ross, H. A. Blacklock, W. Tax, P. E. Thorpe

Research output: Contribution to journalArticle

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Abstract

Four anti-T cell monoclonal antibodies were coupled to ricin-A and tested for their ability to kill T cells in peripheral blood and bone marrow using a clonogenic assay to quantify T cell survival. The immunotoxins (IT) prepared from RFT11 (CD2) and WT1 (CD7) antibodies were the most toxic to peripheral blood T cells. The immunotoxin prepared from RFT1 (CD5) was the next most efficient toxin and the immunotoxin prepared from RFT8 (CD8) was the least toxic. When these reagents were applied to peripheral blood cells at 3 x 10-8 M the number of T cell colonies was reduced by an average of 95%, 94%, 84% and 50%, respectively. Peripheral blood T cells from different donors showed marked variability in their sensitivity to ITs. However, a cocktail of three ITs prepared from RFT11, WT1 and RFT1 gave superior and consistent killing (mean 99.9%; range 99.8-100%) of peripheral blood T cells from six donors. When this cocktail was applied to bone marrow cells from six donors, an average of 99.6% (range 99.5-99.8%) of the T cells were killed. Under the same conditions there was little or no reduction in the number of normal haematopoietic progenitors (CFU-GM, CFU-GEMM, CFU-Meg and BFU-E).

Original languageEnglish (US)
Pages (from-to)407-411
Number of pages5
JournalBritish Journal of Haematology
Volume67
Issue number4
StatePublished - 1987

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Immunotoxins
Bone Marrow
T-Lymphocytes
Blood Cells
Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Poisons
Ricin
Erythroid Precursor Cells
Thomsen-Friedenreich antibodies
Bone Marrow Cells
Cell Survival
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Katz, F. E., Janossy, G., Cumber, A., Ross, W., Blacklock, H. A., Tax, W., & Thorpe, P. E. (1987). Elimination of T cells from human peripheral blood and bone marrow using a cocktail of three anti-T cell immunotoxins. British Journal of Haematology, 67(4), 407-411.

Elimination of T cells from human peripheral blood and bone marrow using a cocktail of three anti-T cell immunotoxins. / Katz, F. E.; Janossy, G.; Cumber, A.; Ross, W.; Blacklock, H. A.; Tax, W.; Thorpe, P. E.

In: British Journal of Haematology, Vol. 67, No. 4, 1987, p. 407-411.

Research output: Contribution to journalArticle

Katz, FE, Janossy, G, Cumber, A, Ross, W, Blacklock, HA, Tax, W & Thorpe, PE 1987, 'Elimination of T cells from human peripheral blood and bone marrow using a cocktail of three anti-T cell immunotoxins', British Journal of Haematology, vol. 67, no. 4, pp. 407-411.
Katz, F. E. ; Janossy, G. ; Cumber, A. ; Ross, W. ; Blacklock, H. A. ; Tax, W. ; Thorpe, P. E. / Elimination of T cells from human peripheral blood and bone marrow using a cocktail of three anti-T cell immunotoxins. In: British Journal of Haematology. 1987 ; Vol. 67, No. 4. pp. 407-411.
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