ELISA for detection of IgG and IgM antibodies to HSV-1 and HSV-2 in human sera

D. Katz, J. K. Hilliard, R. R. Mirkovic, R. A. Word

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A rapid, enzyme-linked immunoassay (ELISA) was applied to identify and measure specific IgG and IgM antibodies to herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2). Detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. Identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with HSV-1 or HSV-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates. Reactivity and the specificity of the reactive immunoglobulin class was quantitated using biotinylated goat anti-IgG and biotinylated goat anti-IgM. Five paired sera from patients with diagnosed herpes simplex genital infections and one human anti-HSV-1 reference serum were tested with this assay and results were compared to results previously obtained using a complement fixation test and micro-SPRIA. The results indicate that the ELISA is a specific, sensitive and simple test which confirms the herpes simplex virus infection history of patients.

Original languageEnglish (US)
Pages (from-to)43-55
Number of pages13
JournalJournal of Virological Methods
Volume14
Issue number1
DOIs
StatePublished - Aug 1986

Keywords

  • ELISA
  • HSV-1
  • HSV-2
  • IgG and IgM
  • antibodies
  • genital herpes

ASJC Scopus subject areas

  • Virology

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