Abstract
A rapid, enzyme-linked immunoassay (ELISA) was applied to identify and measure specific IgG and IgM antibodies to herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2). Detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. Identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with HSV-1 or HSV-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates. Reactivity and the specificity of the reactive immunoglobulin class was quantitated using biotinylated goat anti-IgG and biotinylated goat anti-IgM. Five paired sera from patients with diagnosed herpes simplex genital infections and one human anti-HSV-1 reference serum were tested with this assay and results were compared to results previously obtained using a complement fixation test and micro-SPRIA. The results indicate that the ELISA is a specific, sensitive and simple test which confirms the herpes simplex virus infection history of patients.
Original language | English (US) |
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Pages (from-to) | 43-55 |
Number of pages | 13 |
Journal | Journal of Virological Methods |
Volume | 14 |
Issue number | 1 |
DOIs | |
State | Published - Aug 1986 |
Keywords
- ELISA
- HSV-1
- HSV-2
- IgG and IgM
- antibodies
- genital herpes
ASJC Scopus subject areas
- Virology